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Pathway Description
Methylglyoxal Degradation I
Escherichia coli
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2015-10-14
Last Updated: 2019-08-14
The degradation of methylglyoxal starts with methylglyoxal being degraded by interacting with glutathione and a glyoxalase resulting in the release of a (R)-S-lactoylglutatione. This compound in turn reacts with a water molecule through a glyoxalase II resulting in the releas of glutathione, a hydrogen ion and an R-lactate. The R-lactate in turn reacts with an ubiquinone through a D-lactate dehydrogenase resulting in the release of an ubiquinol and a pyruvate which can then be incorporated the pyruvate metabolism
References
Methylglyoxal Degradation I References
Dym O, Pratt EA, Ho C, Eisenberg D: The crystal structure of D-lactate dehydrogenase, a peripheral membrane respiratory enzyme. Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9413-8.
Pubmed: 10944213
Gonzalez CF, Proudfoot M, Brown G, Korniyenko Y, Mori H, Savchenko AV, Yakunin AF: Molecular basis of formaldehyde detoxification. Characterization of two S-formylglutathione hydrolases from Escherichia coli, FrmB and YeiG. J Biol Chem. 2006 May 19;281(20):14514-22. doi: 10.1074/jbc.M600996200. Epub 2006 Mar 27.
Pubmed: 16567800
Kalapos MP: Methylglyoxal in living organisms: chemistry, biochemistry, toxicology and biological implications. Toxicol Lett. 1999 Nov 22;110(3):145-75.
Pubmed: 10597025
Ozyamak E, Black SS, Walker CA, Maclean MJ, Bartlett W, Miller S, Booth IR: The critical role of S-lactoylglutathione formation during methylglyoxal detoxification in Escherichia coli. Mol Microbiol. 2010 Dec;78(6):1577-90. doi: 10.1111/j.1365-2958.2010.07426.x. Epub 2010 Oct 29.
Pubmed: 21143325
Reiger M, Lassak J, Jung K: Deciphering the role of the type II glyoxalase isoenzyme YcbL (GlxII-2) in Escherichia coli. FEMS Microbiol Lett. 2015 Jan;362(2):1-7. doi: 10.1093/femsle/fnu014. Epub 2014 Dec 4.
Pubmed: 25670698
Escherichia coli and Salmonella: Cellular and Molecular Biology (EcoSal). Online edition.
Clugston SL, Daub E, Kinach R, Miedema D, Barnard JF, Honek JF: Isolation and sequencing of a gene coding for glyoxalase I activity from Salmonella typhimurium and comparison with other glyoxalase I sequences. Gene. 1997 Feb 20;186(1):103-11. doi: 10.1016/s0378-1119(96)00691-9.
Pubmed: 9047352
Clugston SL, Barnard JF, Kinach R, Miedema D, Ruman R, Daub E, Honek JF: Overproduction and characterization of a dimeric non-zinc glyoxalase I from Escherichia coli: evidence for optimal activation by nickel ions. Biochemistry. 1998 Jun 16;37(24):8754-63. doi: 10.1021/bi972791w.
Pubmed: 9628737
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
Pubmed: 9278503
Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. doi: 10.1038/msb4100049. Epub 2006 Feb 21.
Pubmed: 16738553
O'Young J, Sukdeo N, Honek JF: Escherichia coli glyoxalase II is a binuclear zinc-dependent metalloenzyme. Arch Biochem Biophys. 2007 Mar 1;459(1):20-6. doi: 10.1016/j.abb.2006.11.024. Epub 2006 Dec 6.
Pubmed: 17196158
Campbell HD, Rogers BL, Young IG: Nucleotide sequence of the respiratory D-lactate dehydrogenase gene of Escherichia coli. Eur J Biochem. 1984 Oct 15;144(2):367-73. doi: 10.1111/j.1432-1033.1984.tb08473.x.
Pubmed: 6386470
Rule GS, Pratt EA, Chin CC, Wold F, Ho C: Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli. J Bacteriol. 1985 Mar;161(3):1059-68.
Pubmed: 3882663
Kohn LD, Kaback HR: Mechanisms of active transport in isolated bacterial membrane vesicles. XV. Purification and properties of the membrane-bound D-lactate dehydrogenase from Escherichia coli. J Biol Chem. 1973 Oct 25;248(20):7012-7.
Pubmed: 4582730
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