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Pathway Description
Aspartate Metabolism
Saccharomyces cerevisiae
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2015-12-02
Last Updated: 2019-08-14
Aspartate (also named as L-aspartic acid) is an α-amino acid that can be used for protein biosynthesis. Oxalacetic acid is produced from aspartic acid by mitochondrial aspartate aminotransferase, then oxalacetic acid is reduced to malic acid by malate dehydrogenase. Malic acid enters mitochondria through mitochondrial dicarboxylate transporter and forms oxalacetic acid by facilitation of malate dehydrogenase. In the final step, oxalacetic acid is catalyzed by mitochondrial aspartate aminotransferase to form aspartate.
References
Aspartate Metabolism References
Cavero S, Vozza A, del Arco A, Palmieri L, Villa A, Blanco E, Runswick MJ, Walker JE, Cerdan S, Palmieri F, Satrustegui J: Identification and metabolic role of the mitochondrial aspartate-glutamate transporter in Saccharomyces cerevisiae. Mol Microbiol. 2003 Nov;50(4):1257-69.
Pubmed: 14622413
Pastore D, Di Pede S, Passarella S: Isolated durum wheat and potato cell mitochondria oxidize externally added NADH mostly via the malate/oxaloacetate shuttle with a rate that depends on the carrier-mediated transport. Plant Physiol. 2003 Dec;133(4):2029-39. doi: 10.1104/pp.103.028548. Epub 2003 Dec 11.
Pubmed: 14671011
Hung GC, Brown CR, Wolfe AB, Liu J, Chiang HL: Degradation of the gluconeogenic enzymes fructose-1,6-bisphosphatase and malate dehydrogenase is mediated by distinct proteolytic pathways and signaling events. J Biol Chem. 2004 Nov 19;279(47):49138-50. doi: 10.1074/jbc.M404544200. Epub 2004 Sep 8.
Pubmed: 15358789
Steffan JS, McAlister-Henn L: Isolation and characterization of the yeast gene encoding the MDH3 isozyme of malate dehydrogenase. J Biol Chem. 1992 Dec 5;267(34):24708-15.
Pubmed: 1447211
Morin PJ, Subramanian GS, Gilmore TD: AAT1, a gene encoding a mitochondrial aspartate aminotransferase in Saccharomyces cerevisiae. Biochim Biophys Acta. 1992 Dec 29;1171(2):211-4. doi: 10.1016/0167-4781(92)90124-i.
Pubmed: 1482685
Cheret G, Pallier C, Valens M, Diagnan-Fornier B, Fukuhara H, Bolotin-Fukuhara M, Sor F: The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast. Yeast. 1993 Nov;9(11):1259-65. doi: 10.1002/yea.320091113.
Pubmed: 8109175
Dujon B, Alexandraki D, Andre B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, Dusterhoft A, Dusterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzalez A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, Kotter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, Mostl D, Muller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, Pohlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW: Complete DNA sequence of yeast chromosome XI. Nature. 1994 Jun 2;369(6479):371-8. doi: 10.1038/369371a0.
Pubmed: 8196765
Kakhniashvili D, Mayor JA, Gremse DA, Xu Y, Kaplan RS: Identification of a novel gene encoding the yeast mitochondrial dicarboxylate transport protein via overexpression, purification, and characterization of its protein product. J Biol Chem. 1997 Feb 14;272(7):4516-21. doi: 10.1074/jbc.272.7.4516.
Pubmed: 9020177
Johnston M, Hillier L, Riles L, Albermann K, Andre B, Ansorge W, Benes V, Bruckner M, Delius H, Dubois E, Dusterhoft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, Kotter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD, et al.: The nucleotide sequence of Saccharomyces cerevisiae chromosome XII. Nature. 1997 May 29;387(6632 Suppl):87-90.
Pubmed: 9169871
Engel SR, Dietrich FS, Fisk DG, Binkley G, Balakrishnan R, Costanzo MC, Dwight SS, Hitz BC, Karra K, Nash RS, Weng S, Wong ED, Lloyd P, Skrzypek MS, Miyasato SR, Simison M, Cherry JM: The reference genome sequence of Saccharomyces cerevisiae: then and now. G3 (Bethesda). 2014 Mar 20;4(3):389-98. doi: 10.1534/g3.113.008995.
Pubmed: 24374639
Minard KI, McAlister-Henn L: Isolation, nucleotide sequence analysis, and disruption of the MDH2 gene from Saccharomyces cerevisiae: evidence for three isozymes of yeast malate dehydrogenase. Mol Cell Biol. 1991 Jan;11(1):370-80. doi: 10.1128/mcb.11.1.370.
Pubmed: 1986231
Casamayor A, Khalid H, Balcells L, Aldea M, Casas C, Herrero E, Arino J: Sequence analysis of a 13.4 kbp fragment from the left arm of chromosome XV reveals a malate dehydrogenase gene, a putative Ser/Thr protein kinase, the ribosomal L25 gene and four new open reading frames. Yeast. 1996 Sep;12(10B Suppl):1013-20. doi: 10.1002/(SICI)1097-0061(199609)12:10B%3C1013::AID-YEA980%3E3.0.CO;2-5.
Pubmed: 8896265
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonne R, Boyer J, Camasses A, Casamayor A, Casas C, Cheret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K, et al.: The nucleotide sequence of Saccharomyces cerevisiae chromosome XV. Nature. 1997 May 29;387(6632 Suppl):98-102.
Pubmed: 9169874
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Bruckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J, et al.: The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI. Nature. 1997 May 29;387(6632 Suppl):103-5.
Pubmed: 9169875
el Moualij B, Duyckaerts C, Lamotte-Brasseur J, Sluse FE: Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae. Yeast. 1997 May;13(6):573-81. doi: 10.1002/(SICI)1097-0061(199705)13:6<573::AID-YEA107>3.0.CO;2-I.
Pubmed: 9178508
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