Loading Pathway...

Pathway Description

Operon: Cytidyl Pyrophosphate Biosynthesis of Isoprenoids

Escherichia coli

Category:

Metabolite Pathway

Sub-Category:

Signaling

Created: 2015-07-17

Last Updated: 2019-08-16

The ispDF operon in E. coli contains two genes that encode proteins that are used to form building blocks for isoprenoid compounds. There is currently no information on the regulation of this operon. The first gene in the operon, ispD, also known as ygbP, encodes the protein 2-C-methyl-D-erythritol 4-phosphate (MEP) cytidylyltransferase. This protein catalyzes the reaction of MEP, a hydrogen ion and cytidine triphosphate, which form a pyrophosphate molecule and 4-CDP-2-C-methyl-D-erythritol. The second gene, ispF, also known as ygbB or mecS, encodes for the protein 2-C-D-erythritol 2,4-cyclodiphosphate synthase. This protein is involved in the formation of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), which are building blocks in the biosynthesis of isoprenoids, also known as terpenoids.

References

Operon: Cytidyl Pyrophosphate Biosynthesis of Isoprenoids References

Rohdich F, Wungsintaweekul J, Fellermeier M, Sagner S, Herz S, Kis K, Eisenreich W, Bacher A, Zenk MH: Cytidine 5'-triphosphate-dependent biosynthesis of isoprenoids: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl-2-C-methylerythritol. Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11758-63.

Pubmed: 10518523

Englund E, Shabestary K, Hudson EP, Lindberg P: Systematic overexpression study to find target enzymes enhancing production of terpenes in Synechocystis PCC 6803, using isoprene as a model compound. Metab Eng. 2018 Sep;49:164-177. doi: 10.1016/j.ymben.2018.07.004. Epub 2018 Jul 17.

Pubmed: 30025762

Richard SB, Ferrer JL, Bowman ME, Lillo AM, Tetzlaff CN, Cane DE, Noel JP: Structure and mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase. An enzyme in the mevalonate-independent isoprenoid biosynthetic pathway. J Biol Chem. 2002 Mar 8;277(10):8667-72. doi: 10.1074/jbc.C100739200. Epub 2002 Jan 10.

Pubmed: 11786530

Li C, Ichikawa JK, Ravetto JJ, Kuo HC, Fu JC, Clarke S: A new gene involved in stationary-phase survival located at 59 minutes on the Escherichia coli chromosome. J Bacteriol. 1994 Oct;176(19):6015-22. doi: 10.1128/jb.176.19.6015-6022.1994.

Pubmed: 7928962

Herz S, Wungsintaweekul J, Schuhr CA, Hecht S, Luttgen H, Sagner S, Fellermeier M, Eisenreich W, Zenk MH, Bacher A, Rohdich F: Biosynthesis of terpenoids: YgbB protein converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to 2C-methyl-D-erythritol 2,4-cyclodiphosphate. Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2486-90. doi: 10.1073/pnas.040554697.

Pubmed: 10694574

Kemp LE, Bond CS, Hunter WN: Structure of a tetragonal crystal form of Escherichia coli 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase. Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):607-10. doi: 10.1107/s090744490202365x. Epub 2003 Feb 21.

Pubmed: 12595740

Pubmed: 10518523

Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.

Pubmed: 9278503

Highlighted elements will appear in red.

Highlight Compounds

	Magnesium
	Manganese

Highlight Proteins

	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Enter relative concentration values (without units). Elements will be highlighted in a color gradient where red = lowest concentration and green = highest concentration. For the best results, view the pathway in Black and White.

Visualize Compound Data