1638
Pathway
Operon: Carbamoyl Phosphate Synthetase
The carAB operon in E. coli contains two genes which produce the alpha and beta, or small and large chains of the carbamoyl-phosphate synthase protein. This protein is involved in the L-arginine biosynthesis pathway, and because of this, it can be repressed by the arginine repressor protein. This repressor is preesent when arginine is, preventing the biosynthesis of excess arginine.
The first gene in this operon, carA, encodes the alpha or small chain of carbamoyl-phosphate synthase. The second gene, carB, encodes the beta or large chain of the same protein. One of each subunit combines in a heterodimer, which then interact with three other heterodomers to form the final carbamoyl-phosphate synthase protein. This protein is used in the formation of carbamoyl phosphate from glutamine, hydrogen carbonate, ATP and water. This reaction is part of the L-arginine biosynthesis pathway.
Signaling
PW001874
Center
PathwayVisualizationContext2161
1235
1813
#000099
PathwayVisualization1622
1638
Operon: Carbamoyl Phosphate Synthetase
The carAB operon in E. coli contains two genes which produce the alpha and beta, or small and large chains of the carbamoyl-phosphate synthase protein. This protein is involved in the L-arginine biosynthesis pathway, and because of this, it can be repressed by the arginine repressor protein. This repressor is preesent when arginine is, preventing the biosynthesis of excess arginine.
The first gene in this operon, carA, encodes the alpha or small chain of carbamoyl-phosphate synthase. The second gene, carB, encodes the beta or large chain of the same protein. One of each subunit combines in a heterodimer, which then interact with three other heterodomers to form the final carbamoyl-phosphate synthase protein. This protein is used in the formation of carbamoyl phosphate from glutamine, hydrogen carbonate, ATP and water. This reaction is part of the L-arginine biosynthesis pathway.
Signaling
3
279513
6330744
Piette J, Nyunoya H, Lusty CJ, Cunin R, Weyens G, Crabeel M, Charlier D, Glansdorff N, Pierard A: DNA sequence of the carA gene and the control region of carAB: tandem promoters, respectively controlled by arginine and the pyrimidines, regulate the synthesis of carbamoyl-phosphate synthetase in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4134-8.
1638
Pathway
280399
3065519
Charlier D, Weyens G, Roovers M, Piette J, Bocquet C, Pierard A, Glansdorff N: Molecular interactions in the control region of the carAB operon encoding Escherichia coli carbamoylphosphate synthetase. J Mol Biol. 1988 Dec 20;204(4):867-77. doi: 10.1016/0022-2836(88)90047-2.
1638
Pathway
1
Cell
CL:0000000
3
Escherichia coli
562
Prokaryote
1
Cytosol
GO:0005829
5
Cytoplasm
GO:0005737
117
1
3
1
PW_BS000117
94
3
PW_BS000094
101
5
3
1
PW_BS000101
298
Carbamoyl phosphate synthetase small chain
DNA
PW_NA000298
carA
Cps α
137190
117
83
Carbamoyl-phosphate synthase large chain
DNA
PW_NA000083
carB
CPSLC
137193
117
292
ρ-independent terminator
DNA
PW_NA000292
ρIT
137093
117
47
Promoter
DNA
PW_NA000047
P
134701
94
137104
117
5860
Carbamoyl-phosphate synthase large chain
P00968
carB
b0033
AP009048
3
6.3.5.5
137191
117
5989
Carbamoyl-phosphate synthase small chain
P0A6F1
Involved in glutamine catabolic process. 2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
carA
b0032
AP009048
3
6.3.5.5
137192
117
22989
Arginine repressor
P0A6D0
Negatively controls the expression of the four operons of arginine biosynthesis in addition to the carAB operon. Predominantly interacts with A/T residues in ARG boxes. It also binds to a specific site in cer locus. Thus it is essential for cer-mediated site-specific recombination in ColE1. It is necessary for monomerization of the plasmid ColE1.
argR
25
137194
101
137196
117
1385
carbamoyl phosphate synthetase
3
PW_P001385
1615
5860
2
1616
5989
2
13600
Arginine repressor
3
PW_P013600
23472
22989
137195
101
180497
PW_R180497
Right
681475
298
1
NucleicAcid
false
681476
1385
1
ProteinComplex
false
180498
PW_R180498
Right
681477
83
1
NucleicAcid
false
681478
1385
1
ProteinComplex
false
180499
PW_R180499
Right
681479
83
1
NucleicAcid
false
681480
292
1
NucleicAcid
false
180500
PW_R180500
Right
681481
47
1
NucleicAcid
false
681482
298
1
NucleicAcid
false
2958
Inhibition
PW_I002958
5915
13600
ProteinComplex
1
5916
47
NucleicAcid
1
3921
298
225
117
false
520
583
14
142
gene
regular
150
30
3922
83
226
117
false
660
583
14
139
gene
regular
150
30
3923
292
229
117
false
799
583
14
150
nucleic_acid
regular
150
30
3924
47
229
117
false
370
583
14
150
nucleic_acid
regular
150
30
952102
5860
117
6
false
650
798
8
subunit
regular
160
80
952103
5989
117
6
false
510
798
8
subunit
regular
160
80
952106
22989
117
2
false
370
383
8
subunit
regular
150
70
806089
1385
1622
117
947867
952102
947868
952103
806092
13600
1622
117
947871
952106
3674862
M591 613 C591 643 525 618 525 648
5
true
18
3674863
M590 798 C590 768 591 643 591 613
5
false
18
true
M 1047.9468550441647 363.261556296296 L 1033 362 L 1039.380887721186 375.5751343230783
false
3674864
M729.5 613 C729.5 643 895 635.5 895 665.5
5
true
18
3674865
M730 798 C730 768 729.5 643 729.5 613
5
false
18
true
M 1047.9468550441647 363.261556296296 L 1033 362 L 1039.380887721186 375.5751343230783
false
3674866
M799 598 C829 598 931.75 523 961.75 523
5
true
18
3674867
M799 598 C769 598 991.75 523 961.75 523
5
true
18
true
M 887.9468550441649 363.261556296296 L 873 362 L 879.3808877211858 375.5751343230783
false
3674868
M520 598 C550 598 498 523 528 523
5
true
18
3674869
M520 598 C490 598 558 523 528 523
5
true
18
true
M 887.9468550441649 363.261556296296 L 873 362 L 879.3808877211858 375.5751343230783
false
3674874
M445 583 C445 553 445 528 445 498
148
true
18
false
true
M 124.56596732297899 -0.31357802678230584 L 116 12 L 107.43403267702101 24.313578026782306
3674875
M445 453 C445 483 445 553 445 583
148
false
18
false
true
M 1207 745 L 1222 745 L 1237 745
800204
1622
180497
117
1841
3921
3674862
Left
18952
806089
3674863
Right
800205
1622
180498
117
1842
3922
3674864
Left
18953
806089
3674865
Right
800206
1622
180499
117
1843
3922
3674866
Left
1844
3923
3674867
Right
800207
1622
180500
117
1845
3924
3674868
Left
1846
3921
3674869
Right
7936
2958
1622
117
40
3924
3674874
Right
9377
806092
3674875
Left
1281
1013
409
0.9
0.9
0
2
92
479
481
3165
M274 376 C274 326 324 276 374 276 C694 276 1109 276 1429 276 C1479 276 1529 326 1529 376 C1529 523 1529 714 1529 861 C1529 911 1479 961 1429 961 C1109 961 694 961 374 961 C324 961 274 911 274 861 C274 714 274 523 274 376
1
true
6
1255.0
685.0
3166
M125 225 C125 175 175 125 225 125 C638 125 1175 125 1588 125 C1638 125 1688 175 1688 225 C1688 463 1688 772 1688 1010 C1688 1060 1638 1110 1588 1110 C1175 1110 638 1110 225 1110 C175 1110 125 1060 125 1010 C125 772 125 463 125 225
1
true
6
1563.0
985.0