PathWhiz

Pathway Description

Methionine Metabolism

Arabidopsis thaliana

Metabolic Pathway

The methionine metabolism starts from aspartate-produced homoserine. Homoserine reacts with HSK resulting in the release of O-phospho-L-homoserine. The latter compound interacts with cysteine through CGS resulting in the release of phosphate and cystathionine. The latter compound reacts with COI3 resulting in the release of 2-aminoprop-2-enoate, hydrogen ion and homocysteine. Homocysteine can react with S-adenosyl-L-methionine through a HMT protein complex resulting in the release of methionine. Methionine can be used to generate S-adenosyl-L-methionine or it can generate oxobutanoate

References

Methionine Metabolism References

Curien G, Ravanel S, Robert M, Dumas R: Identification of six novel allosteric effectors of Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase isoforms. Physiological context sets the specificity. J Biol Chem. 2005 Dec 16;280(50):41178-83. doi: 10.1074/jbc.M509324200. Epub 2005 Oct 10.

Pubmed: 16216875

Frankard V, Vauterin M, Jacobs M: Molecular characterization of an Arabidopsis thaliana cDNA coding for a monofunctional aspartate kinase. Plant Mol Biol. 1997 May;34(2):233-42.

Pubmed: 9207839

Jander G, Joshi V: Aspartate-Derived Amino Acid Biosynthesis in Arabidopsis thaliana. Arabidopsis Book. 2009;7:e0121. doi: 10.1199/tab.0121. Epub 2009 Jun 10.

Pubmed: 22303247

Kim J, Lee M, Chalam R, Martin MN, Leustek T, Boerjan W: Constitutive overexpression of cystathionine gamma-synthase in Arabidopsis leads to accumulation of soluble methionine and S-methylmethionine. Plant Physiol. 2002 Jan;128(1):95-107.

Pubmed: 11788756

Ranocha P, Bourgis F, Ziemak MJ, Rhodes D, Gage DA, Hanson AD: Characterization and functional expression of cDNAs encoding methionine-sensitive and -insensitive homocysteine S-methyltransferases from Arabidopsis. J Biol Chem. 2000 May 26;275(21):15962-8. doi: 10.1074/jbc.M001116200.

Pubmed: 10747987

Ravanel S, Gakiere B, Job D, Douce R: Cystathionine gamma-synthase from Arabidopsis thaliana: purification and biochemical characterization of the recombinant enzyme overexpressed in Escherichia coli. Biochem J. 1998 Apr 15;331 ( Pt 2):639-48.

Pubmed: 9531508

Gakiere B, Job D, Douce R, Ravanel S: Characterization of the cDNA and Gene for a Cytosolic Cobalamin-Independent Methionine Synthase in Arabidopsis thaliana. Journal of Plant Physiology. 1999;120:1206. doi: 10.1104/pp.120.4.1205.

Jones PR, Manabe T, Awazuhara M, Saito K: A new member of plant CS-lyases. A cystine lyase from Arabidopsis thaliana. J Biol Chem. 2003 Mar 21;278(12):10291-6. doi: 10.1074/jbc.M212207200. Epub 2003 Jan 13.

Pubmed: 12525491

Lee M, Martin MN, Hudson AO, Lee J, Muhitch MJ, Leustek T: Methionine and threonine synthesis are limited by homoserine availability and not the activity of homoserine kinase in Arabidopsis thaliana. Plant J. 2005 Mar;41(5):685-96. doi: 10.1111/j.1365-313X.2004.02329.x.

Pubmed: 15703056

Lee M, Leustek T: Identification of the gene encoding homoserine kinase from Arabidopsis thaliana and characterization of the recombinant enzyme derived from the gene. Arch Biochem Biophys. 1999 Dec 1;372(1):135-42. doi: 10.1006/abbi.1999.1481.

Pubmed: 10562426

Ravanel S, Job D, Douce R: Purification and properties of cystathionine beta-lyase from Arabidopsis thaliana overexpressed in Escherichia coli. Biochem J. 1996 Dec 1;320 ( Pt 2):383-92.

Pubmed: 8973544

Rebeille F, Jabrin S, Bligny R, Loizeau K, Gambonnet B, Van Wilder V, Douce R, Ravanel S: Methionine catabolism in Arabidopsis cells is initiated by a gamma-cleavage process and leads to S-methylcysteine and isoleucine syntheses. Proc Natl Acad Sci U S A. 2006 Oct 17;103(42):15687-92. doi: 10.1073/pnas.0606195103. Epub 2006 Oct 9.

Pubmed: 17030798

Enter relative concentration values (without units). Elements will be highlighted in a color gradient where red = lowest concentration and green = highest concentration. For the best results, view the pathway in Black and White.

Visualize Compound Data

		(2Z)-2-aminobut-2-enoate
		2-aminoprop-2-enoate
		2-iminobutanoate
		2-Ketobutyric acid
		Adenosine diphosphate
		Adenosine triphosphate
		Ammonium
		Homocysteine
		Hydrogen Ion
		L-Aspartate-semialdehyde
		L-Aspartic acid
		L-Aspartyl-4-phosphate
		L-Cystathionine
		L-Cysteine
		L-Homoserine
		L-Methionine
		Methanethiol
		NADP
		NADPH
		O-Phosphohomoserine
		Phosphate
		Pyrophosphate
		S-Adenosylhomocysteine
		S-Adenosylmethionine
		Water

Visualize Protein Data

		Aspartate semialdehyde dehydrogenase
		Aspartokinase 1, chloroplastic
		Aspartokinase 2, chloroplastic
		Aspartokinase 3, chloroplastic
		Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic
		Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic
		Cystathionine gamma-synthase 1, chloroplastic
		Cystine lyase CORI3
		Homocysteine S-methyltransferase 1
		Homocysteine S-methyltransferase 2
		Homoserine kinase
		Methionine gamma-lyase
		S-adenosylmethionine synthase 1

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