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Gq protein signaling cascade Phospholipase C Protein kinase C alpha type Myosin light chain kinase, smooth muscle Histamine H1 receptor Voltage- dependent P/Q-type calcium channel subunit alpha-1A Voltage- dependent L-type calcium channel subunit beta-1 Voltage- dependent calcium channel subunit alpha-2/delta-2 Inositol 1,4,5- trisphosphate receptor type 1 Myosin LC-P Myosin light chain 3 Calmodulin-1 Myosin light chain phosphatase Calmodulin-1 Histamine Inositol 1,4,5- trisphosphate Antazoline Ca+ Ca+ Ca+ Antazoline Phosphatidylinositol 4,5-bisphosphate Calcium Ca+ Diacylglycerol Mast Cells Release Histamine Muscle Contraction Muscle Relaxation Myosin Magnesium Calcium Manganese Sarcoplasmic Reticulum Calcium cannot bind to calmodulin to activate myosin light chain kinase Myosin light chain kinase is not activated which leads to accumulation of myosin light chain 3 causing muscle relaxation There is an overall decrease in calcium levels in the cytosol caused by the inhibition of the Gq signaling cascade Cytosol Bronchiole Myocyte Antazoline antagonizes Histamine H1 receptors in smooth muscles, preventing histamine from activating the receptor Actin Actin and myosin bind bringing the filaments together causing muscle contraction Lungs Bronchioles
GNAQ PLCB1 PRKCA MYLK HRH1 CACNA1A CACNB1 CACNA2D2 ITPR1 MYL3 MYL3 CALM1 PPP1CB CALM1 Histamine Inositol 1,4,5- trisphosphate Antazoline Calcium Calcium Calcium Antazoline Phosphatidylinositol 4,5-bisphosphate Calcium Diacylglycerol Mast Cells Release Histamine Muscle Contraction Muscle Relaxation
GNAQ PLCB1 PRKCA MYLK HRH1 CACNA1A CACNB1 CACNA2D2 ITPR1 MYL3 MYL3 CALM1 PPP1CB CALM1 Hsm Inotp Antazo Ca+ Ca+ Ca+ Antazo P45P Ca2+ Ca+ Diacylg M C R H Mus Con Mus Rel Myosin Mg2+ Ca2+ Mn2+ Sarcoplasmic Reticulum Calcium cannot bind to calmodulin to activate myosin light chain kinase Myosin light chain kinase is not activated which leads to accumulation of myosin light chain 3 causing muscle relaxation There is an overall decrease in calcium levels in the cytosol caused by the inhibition of the Gq signaling cascade Cytosol Bronchiole Myocyte Antazoline antagonizes Histamine H1 receptors in smooth muscles, preventing histamine from activating the receptor Actin Actin and myosin bind bringing the filaments together causing muscle contraction Lungs Bronchioles
GNAQ PLCB1 PRKCA MYLK HRH1 CACNA1A CACNB1 CACNA2D2 ITPR1 MYL3 MYL3 CALM1 PPP1CB CALM1 Hsm Inotp Antazo Ca2+ Ca2+ Ca2+ Antazo P45P Ca2+ Diacylg M C R H Mus Con Mus Rel