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Gq protein signaling cascade Phospholipase C Protein kinase C alpha type Histamine H1 receptor Nitric oxide synthase, endothelial Myosin light chain phosphatase Voltage- dependent P/Q-type calcium channel subunit alpha-1A Voltage- dependent L-type calcium channel subunit beta-1 Voltage- dependent calcium channel subunit alpha-2/delta-2 Inositol 1,4,5- trisphosphate receptor type 1 Myosin LC-P Myosin light chain 3 Calmodulin-1 Myosin light chain kinase, smooth muscle Calmodulin-1 Histamine NO Inositol 1,4,5- trisphosphate Benzquinamide NO Ca+ Ca+ Ca+ Benzquinamide Phosphatidylinositol 4,5-bisphosphate L-Arginine H+ NADPH O2 H2O Citrulline NADP Calcium Heme FAD Flavin Mononucleotide Tetrahydrobiopterin Ca+ Diacylglycerol Muscle Contraction Muscle relaxation Mast Cells Release Histamine Manganese Magnesium Calcium Endoplasmic Reticulum Calcium cannot bind to calmodulin to activate the calmodulin-binding domain of nitric oxide synthase. There is an overall decrease in calcium levels in the cytosol caused by the inhibition of the Gq signaling cascade Cytosol Endothelial Cell Benzquinamide antagonizes Histamine H1 receptors in endothelial cells, preventing histamine from activating the receptor The inhibition of Nitric oxide synthesis prevents the activation of myosin light chain phosphatase. This causes an accumulation of myosin light chain-phosphate which causes the muscle to contract. Myocyte Actin and myosin bind bringing the filaments together causing muscle contraction Actin filament Myosin filament Blood Vessel constriction Blood vessel
GNAQ PLCB1 PRKCA HRH1 NOS3 PPP1CB CACNA1A CACNB1 CACNA2D2 ITPR1 MYL3 MYL3 CALM1 MYLK CALM1 Histamine Nitric oxide Inositol 1,4,5- trisphosphate Benzquinamide Nitric oxide Calcium Calcium Calcium Benzquinamide Phosphatidylinositol 4,5-bisphosphate L-Arginine Hydrogen Ion NADPH Oxygen Water Citrulline NADP Calcium Diacylglycerol Muscle Contraction Muscle relaxation Mast Cells Release Histamine
GNAQ PLCB1 PRKCA HRH1 NOS3 PPP1CB CACNA1A CACNB1 CACNA2D2 ITPR1 MYL3 MYL3 CALM1 MYLK CALM1 Hsm NO Inotp Bzq NO Ca+ Ca+ Ca+ Bzq P45P Arg H+ NADPH O2 H2O Citruln NADP Ca2+ Heme FAD FlvnMnt BH4 Ca+ Diacylg Mus Con Mus rel M C R H Mn2+ Mg2+ Ca2+ Endoplasmic Reticulum Calcium cannot bind to calmodulin to activate the calmodulin-binding domain of nitric oxide synthase. There is an overall decrease in calcium levels in the cytosol caused by the inhibition of the Gq signaling cascade Cytosol Endothelial Cell Benzquinamide antagonizes Histamine H1 receptors in endothelial cells, preventing histamine from activating the receptor The inhibition of Nitric oxide synthesis prevents the activation of myosin light chain phosphatase. This causes an accumulation of myosin light chain-phosphate which causes the muscle to contract. Myocyte Actin and myosin bind bringing the filaments together causing muscle contraction Actin filament Myosin filament Blood Vessel constriction Blood vessel
GNAQ PLCB1 PRKCA HRH1 NOS3 PPP1CB CACNA1A CACNB1 CACNA2D2 ITPR1 MYL3 MYL3 CALM1 MYLK CALM1 Hsm NO Inotp Bzq NO Ca2+ Ca2+ Ca2+ Bzq P45P Arg H+ NADPH O2 H2O Citruln NADP Ca2+ Diacylg Mus Con Mus rel M C R H