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Pathway Description
Secondary Metabolites: Glyoxylate Cycle
Escherichia coli
Metabolic Pathway
The glyoxylate cycle starts with the interaction of Acetyl-Coa with a water molecule and Oxalacetic acid interact through a Citrate synthase resulting in a release of a coenzyme a and citric acid. The citric acid gets dehydrated through a citrate hydro-lyase resulting in the release of a water molecule and cis-Aconitic acid. The cis-Aconitic acid is then hydrated in an reversible reaction through an aconitate hydratase resulting in an Isocitric acid. The isocitric acid then interacts in a reversible reaction through isocitrate lyase resulting in the release of a succinic acid and a glyoxylic acid. The glyoxylic acid then reacts in a reversible reaction with an acetyl-coa, and a water molecule in a reversible reaction, resulting in a release of a coenzyme A, a hydrogen ion and an L-malic acid. The L-malic acid interacts in a reversible reaction through a NAD driven malate dehydrogenase resulting in the release of NADH, a hydrogen ion and an Oxalacetic acid.
References
Secondary Metabolites: Glyoxylate Cycle References
Cortay JC, Bleicher F, Duclos B, Cenatiempo Y, Gautier C, Prato JL, Cozzone AJ: Utilization of acetate in Escherichia coli: structural organization and differential expression of the ace operon. Biochimie. 1989 Sep-Oct;71(9-10):1043-9.
Pubmed: 2512996
LaPorte DC, Walsh K, Koshland DE Jr: The branch point effect. Ultrasensitivity and subsensitivity to metabolic control. J Biol Chem. 1984 Nov 25;259(22):14068-75.
Pubmed: 6389540
Nimmo GA, Nimmo HG: The regulatory properties of isocitrate dehydrogenase kinase and isocitrate dehydrogenase phosphatase from Escherichia coli ML308 and the roles of these activities in the control of isocitrate dehydrogenase. Eur J Biochem. 1984 Jun 1;141(2):409-14.
Pubmed: 6329757
Walsh K, Koshland DE Jr: Determination of flux through the branch point of two metabolic cycles. The tricarboxylic acid cycle and the glyoxylate shunt. J Biol Chem. 1984 Aug 10;259(15):9646-54.
Pubmed: 6378912
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