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Pathway Description
Secondary Metabolites: Trehalose Biosynthesis and Metabolism
Escherichia coli
Metabolic Pathway
Threhalose biosynthesis begins with an Alpha-D-glucose-1-phosphate interacting with an ATP through a glucose-1-phosphate adenylyltransferase resulting in the release of a pyrophosphate and an ADP-glucose. The latter compound interacts in a reversible reaction with an amylose through a glycogen synthase resulting in the release of an ADP and an amylose. Amylose then interacts in a reversible reaction with 1,4-α-glucan branching enzyme resulting in a glycogen
Glycogen can also be produced by a reversible reaction with Amylose through a maltodextrin phosphorylase, releasing a phosphate and a glycogen.
Glycogen is then transformed into trehalose through a glycogen debranching enzyme. Alpha Alpha Trehalose can be degraded by reacting with with a water molecule through a cytoplasmic trehalase resulting in the release of a Beta-D-glucose and an Alpha-D-glucose.phosphorylated resulting in a Beta-D-glucose 6-phosphate. This compound is phosphorylated and can then join glycolysis
Alpha Alpha Trehalose can be degraded in the periplasmic space by reacting with with a water molecule through a periplasmic trehalase resulting in the release of a Beta-D-glucose and an Alpha-D-glucose.
The beta-D-glucose can be transported into the cytosol through a PTS permease where it is phosphorylated resulting in a Beta-D-glucose 6-phosphate. This compound can then join glycolysis
References
Secondary Metabolites: Trehalose Biosynthesis and Metabolism References
Arguelles JC: Physiological roles of trehalose in bacteria and yeasts: a comparative analysis. Arch Microbiol. 2000 Oct;174(4):217-24.
Pubmed: 11081789
Elbein AD, Pan YT, Pastuszak I, Carroll D: New insights on trehalose: a multifunctional molecule. Glycobiology. 2003 Apr;13(4):17R-27R. doi: 10.1093/glycob/cwg047. Epub 2003 Jan 22.
Pubmed: 12626396
Giaever HM, Styrvold OB, Kaasen I, Strom AR: Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli. J Bacteriol. 1988 Jun;170(6):2841-9.
Pubmed: 3131312
Hengge-Aronis R, Klein W, Lange R, Rimmele M, Boos W: Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary-phase thermotolerance in Escherichia coli. J Bacteriol. 1991 Dec;173(24):7918-24.
Pubmed: 1744047
Horlacher R, Peist R, Boos W: Improved method for the preparative synthesis of labeled trehalose of high specific activity by Escherichia coli. Appl Environ Microbiol. 1996 Oct;62(10):3861-3.
Pubmed: 8837441
Kandror O, DeLeon A, Goldberg AL: Trehalose synthesis is induced upon exposure of Escherichia coli to cold and is essential for viability at low temperatures. Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9727-32. doi: 10.1073/pnas.142314099. Epub 2002 Jul 8.
Pubmed: 12105274
Larsen PI, Sydnes LK, Landfald B, Strom AR: Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose. Arch Microbiol. 1987 Feb;147(1):1-7.
Pubmed: 2883950
Richards AB, Krakowka S, Dexter LB, Schmid H, Wolterbeek AP, Waalkens-Berendsen DH, Shigoyuki A, Kurimoto M: Trehalose: a review of properties, history of use and human tolerance, and results of multiple safety studies. Food Chem Toxicol. 2002 Jul;40(7):871-98.
Pubmed: 12065209
Ruhal R, Kataria R, Choudhury B: Trends in bacterial trehalose metabolism and significant nodes of metabolic pathway in the direction of trehalose accumulation. Microb Biotechnol. 2013 Sep;6(5):493-502. doi: 10.1111/1751-7915.12029. Epub 2013 Jan 10.
Pubmed: 23302511
Styrvold OB, Strom AR: Synthesis, accumulation, and excretion of trehalose in osmotically stressed Escherichia coli K-12 strains: influence of amber suppressors and function of the periplasmic trehalase. J Bacteriol. 1991 Feb;173(3):1187-92.
Pubmed: 1825082
Strom AR, Kaasen I: Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression. Mol Microbiol. 1993 Apr;8(2):205-10.
Pubmed: 8391102
Horlacher R, Uhland K, Klein W, Ehrmann M, Boos W: Characterization of a cytoplasmic trehalase of Escherichia coli. J Bacteriol. 1996 Nov;178(21):6250-7.
Pubmed: 8892826
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