Loading Pathway...
Error: Pathway image not found.
Hide
Pathway Description
Operon: tRNA Synthetase & Peptidase
Escherichia coli
Signaling Pathway
The ribF-ileS-lspA-fkpB-ispH operon in E. coli contains five genes that encode for various proteins. There are currently no known activators and repressors of this operon.
The first gene, ribF, encodes for a bifunctional riboflavin kinase and FMN adenylyltransferase. In the cell, it is used to convert riboflavin to FMN, and then FMN to FAD.
The second gene, ileS, encodes the isoleucine tRNA ligase or synthetase, which catalyzes the attachment of the amimno acid isoleucine onto its tRNA, forming a charged tRNA.
The third gene, lspA, encodes a lipoprotein signal peptidase. This protein cleaves signal peptides from prolipoproteins, forming the mature lipoprotein, as well as a free signal peptide which is then degraded.
The fourth gene, fkpB, encodes a peptidyl-prolyl cis-trans isomerase which works as an isomerase, and also posesses a chaperone activity, assisting in the folding of several ribosomal proteins.
The final gene, ispH, encodes 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) reductase. This protein catalyzes the conversion of HMBPP into both dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP) as part of the methylerythritol phosphate pathway involved in isoprenoid biosynthesis.
References
Operon: tRNA Synthetase & Peptidase References
Kamio Y, Lin CK, Regue M, Wu HC: Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon. J Biol Chem. 1985 May 10;260(9):5616-20.
Pubmed: 2985604
Goh S, Hohmeier A, Stone TC, Offord V, Sarabia F, Garcia-Ruiz C, Good L: Silencing of Essential Genes within a Highly Coordinated Operon in Escherichia coli. Appl Environ Microbiol. 2015 Aug 15;81(16):5650-9. doi: 10.1128/AEM.01444-15. Epub 2015 Jun 12.
Pubmed: 26070674
Ross, W., Vrentas, C.E., Sanchez-Vazquez, P., Gaal, T. and Gourse, R.L., 2013. The magic spot: a ppGpp binding site on E. coli RNA polymerase responsible for regulation of transcription initiation. Molecular cell, 50(3), pp.420-429.
Zuo, Y., Wang, Y. and Steitz, T.A., 2013. The mechanism of E. coli RNA polymerase regulation by ppGpp is suggested by the structure of their complex. Molecular cell, 50(3), pp.430-436.
Highlighted elements will appear in red.
Highlight Compounds
Highlight Proteins
Enter relative concentration values (without units). Elements will be highlighted in a color gradient where red = lowest concentration and green = highest concentration. For the best results, view the pathway in Black and White.
Visualize Compound Data
Visualize Protein Data
Downloads
Settings