PathWhiz

Pathway Description

Purine Degradation

Escherichia coli

Metabolic Pathway

Pseudouridine is phosphorylated by interacting with atp and a psuK resulting in the release of an ADP, a hydrogen ion and a pseudouridine 5'-phosphate. The latter compound then reacts with water through a pseudouridine 5'-phosphate glycosidase resulting in the release of a uracil and D-ribofuranose 5-phosphate

References

Purine Degradation References

Breitman TR, Scher CD: Growth of pyrimidineless strains of Escherichia coli on 5-ribosyluracil (pseudouridine). J Bacteriol. 1968 Nov;96(5):1873-4.

Pubmed: 4882030

Breitman TR: Pseudouridulate synthetase of Escherichia coli: correlation of its activity with utilization of pseudouridine for growth. J Bacteriol. 1970 Jul;103(1):263-4.

Pubmed: 4912525

Del Campo M, Kaya Y, Ofengand J: Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli. RNA. 2001 Nov;7(11):1603-15.

Pubmed: 11720289

Ferre-D'Amare AR: RNA-modifying enzymes. Curr Opin Struct Biol. 2003 Feb;13(1):49-55.

Pubmed: 12581659

Huang L, Pookanjanatavip M, Gu X, Santi DV: A conserved aspartate of tRNA pseudouridine synthase is essential for activity and a probable nucleophilic catalyst. Biochemistry. 1998 Jan 6;37(1):344-51. doi: 10.1021/bi971874+.

Pubmed: 9425056

Preumont A, Snoussi K, Stroobant V, Collet JF, Van Schaftingen E: Molecular identification of pseudouridine-metabolizing enzymes. J Biol Chem. 2008 Sep 12;283(37):25238-46. doi: 10.1074/jbc.M804122200. Epub 2008 Jun 30.

Pubmed: 18591240

Ramamurthy V, Swann SL, Paulson JL, Spedaliere CJ, Mueller EG: Critical aspartic acid residues in pseudouridine synthases. J Biol Chem. 1999 Aug 6;274(32):22225-30.

Pubmed: 10428788

Solomon LR, Breitman TR: Pseudouridine kinase of escherichia coli: a new enzyme. Biochem Biophys Res Commun. 1971 Jul 16;44(2):299-304.

Pubmed: 4334133

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