The biosynthesis of tetrahydrofolate begins with guanosine triphosphate interacting with water through GTP-cyclohydrlase resulting in the release of a formic acid, a hydrogen ion and a dihydroneopterin triphosphate. The latter compound then reacts with water in a spontaneous reaction resulting in the release of pyrophosphate, hydrogen ion and dihydroneopterinphosphate. Dihydroneopterin phosphate then reacts spontaneously with water resulting in the release of phosphate and 7,8-dihydroneopterin. This compound reacts wuth a folic acid synthesis enzyme resulting in the release of glycoaldehyde and 6-hydroxymethyl-7,8-dihydropterin. The latter compound is then diphosphorylated through an ATP driven folic acid synthesis resulting in the release of AMP, a hydrogen ion and 6-hydroxymethyl-7,8-dihydropterin diphosphate. This compound reacts with p-Aminobenzoic acid that is release from chorismate, the reaction happens through a folic acid synthesis resulting in the pyrophosphate and 7,8-dihydropteric acid. The latter compound reacts with glutamic acid through an ATP driven folic acid synthesis 3 resulting in the release of hydrogen ion, a phosphate, ADP and a 7,8-dihydrofolate monoglutamate. The latter compound reacts with a hydrogen ion through a NADPH through a dihydrofolate reductase resulting in the release of NADP and tetrahydrofolate. This compound can also be a result of 5,10 methenyltetrahydrofolic acid reacting with water through a mitochondrials c1-tetrahydrofolate synthase which releases a 10-formyltetrahydrofolate. This compound in turn reacts with a 5-phosphoribosyl-N-formylglycinamide through a glycinamide ribotide transformylase resulting in the release of a tetrahydrofolate and a 5'phosphoribosyl-N-fromylglycinamide.

Tetrahydrofolate Biosynthesis References