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Pathway Description
N-Glycan Biosynthesis
Saccharomyces cerevisiae
Metabolic Pathway
The N-glycan biosynthesis is a pathway involving the creation of a dolichol, and the consecutive reactions involving the addition of Acetylglucosaminyl groups, mannosyl groups and glucosyl groups. The set of reactions all happen in the ER membrane. The resulting glucosyl3mannosyl9-N-acetylglucosaminyl2-diphosphodolichol is used as protein modificator.
References
N-Glycan Biosynthesis References
Bickel T, Lehle L, Schwarz M, Aebi M, Jakob CA: Biosynthesis of lipid-linked oligosaccharides in Saccharomyces cerevisiae: Alg13p and Alg14p form a complex required for the formation of GlcNAc(2)-PP-dolichol. J Biol Chem. 2005 Oct 14;280(41):34500-6. doi: 10.1074/jbc.M506358200. Epub 2005 Aug 12.
Pubmed: 16100113
Burda P, Aebi M: The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta. 1999 Jan 6;1426(2):239-57.
Pubmed: 9878760
Burda P, Jakob CA, Beinhauer J, Hegemann JH, Aebi M: Ordered assembly of the asymmetrically branched lipid-linked oligosaccharide in the endoplasmic reticulum is ensured by the substrate specificity of the individual glycosyltransferases. Glycobiology. 1999 Jun;9(6):617-25.
Pubmed: 10336995
Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N: The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum. J Biol Chem. 2001 Jun 15;276(24):21828-40. doi: 10.1074/jbc.M010896200. Epub 2001 Feb 15.
Pubmed: 11278778
Herscovics A, Orlean P: Glycoprotein biosynthesis in yeast. FASEB J. 1993 Apr 1;7(6):540-50.
Pubmed: 8472892
Jakob CA, Burda P, Roth J, Aebi M: Degradation of misfolded endoplasmic reticulum glycoproteins in Saccharomyces cerevisiae is determined by a specific oligosaccharide structure. J Cell Biol. 1998 Sep 7;142(5):1223-33.
Pubmed: 9732283
Kukuruzinska MA, Bergh ML, Jackson BJ: Protein glycosylation in yeast. Annu Rev Biochem. 1987;56:915-44. doi: 10.1146/annurev.bi.56.070187.004411.
Pubmed: 3304149
Lehle L, Strahl S, Tanner W: Protein glycosylation, conserved from yeast to man: a model organism helps elucidate congenital human diseases. Angew Chem Int Ed Engl. 2006 Oct 20;45(41):6802-18. doi: 10.1002/anie.200601645.
Pubmed: 17024709
Moremen KW, Trimble RB, Herscovics A: Glycosidases of the asparagine-linked oligosaccharide processing pathway. Glycobiology. 1994 Apr;4(2):113-25.
Pubmed: 8054711
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