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Cytochrome P450 90B1 3-epi-6- deoxocathasterone 23- monooxygenase CYP90D1 Cytochrome P450 90A1 Steroid 5-alpha- reductase DET2 3-epi-6- deoxocathasterone 23- monooxygenase CYP90D1 Steroid 5-alpha- reductase DET2 Cytochrome P450 90B1 3-epi-6- deoxocathasterone 23- monooxygenase CYP90D1 Cytochrome P450 90B1 3-epi-6- deoxocathasterone 23- monooxygenase CYP90D1 3-epi-6- deoxocathasterone 23- monooxygenase CYP90D1 Cytochrome P450 85A2 Cytochrome P450 85A2 Cytochrome P450 90D2 Cytochrome P450 90D2 3-epi-6- deoxocathasterone 23- monooxygenase CYP90D1 Cytochrome P450 85A2 Cytochrome P450 90B1 Cytochrome P450 85A2 Cytochrome P450 85A2 Chaperonin CPN60-like 1, mitochondrial Chaperonin CPN60-like 1, mitochondrial Cytochrome P450 85A2 Cytochrome P450 85A2 Cytochrome P450 734A1 Cytochrome P450 85A2 Cytochrome P450 734A1 Campesterol 22alpha- Hydroxycampesterol O2 FMNH2 (22R,23R)- 22,23- Dihydroxycampesterol FMN H2O H+ (22α)- hydroxy- campest-4-en- 3-one H+ NADPH (22α)- hydroxy-5α- campestan-3- one NADP 3-Epi-6- deoxocathesterone O2 FMNH2 FMN H+ H2O 6- Deoxotyphasterol Campest-4-en- 3beta-ol Campest-4-en- 3-one H+ NADPH (5α)- campestan-3- one NADP (22R, 23R)-22,23- dihydroxy- campest-4-en- 3-one FMNH2 O2 H+ H2O FMN Campestanol 6a- hydroxycampestanol 6- Oxocampestanol 6- Deoxocathasterone Cathasterone O2 FMNH2 Teasterone H2O H+ FMN O2 FMNH2 6- Deoxoteasterone H+ FMN H2O 7-Oxateasterone 3- Dehydroteasterone 3-Dehydro-6- deoxo- teasterone H+ H2O FMN O2 FMNH2 2- Deoxycastasterone 7- Oxatyphasterol Castasterone 6- Deoxocastasterone 6a- Hydroxycastasterone 26- Hydroxycastasterone Brassinolide 26- Hydroxybrassinolide Steroid Biosynthesis Mitochondrial Matrix Endoplasmic Reticulum Endoplasmic Reticulum
Endoplasmic Reticulum Endoplasmic Reticulum Mitochondria CYP90B1 CYP90D1 CYP90A1 DET2 CYP90D1 DET2 CYP90B1 CYP90D1 CYP90B1 CYP90D1 CYP90D1 CYP85A2 CYP85A2 CYP90D2 CYP90D2 CYP90D1 CYP85A2 CYP90B1 CYP85A2 CYP85A2 At2g33210 At2g33210 CYP85A2 CYP85A2 CYP734A1 CYP85A2 CYP734A1 Campesterol 22alpha- Hydroxycampesterol Oxygen FMNH2 (22R,23R)- 22,23- Dihydroxycampesterol FMN Water Hydrogen Ion (22α)- hydroxy- campest-4-en- 3-one Hydrogen Ion NADPH (22α)- hydroxy-5α- campestan-3- one NADP 3-Epi-6- deoxocathesterone Oxygen FMNH2 FMN Hydrogen Ion Water 6- Deoxotyphasterol Campest-4-en- 3beta-ol Campest-4-en- 3-one Hydrogen Ion NADPH (5α)- campestan-3- one NADP (22R, 23R)-22,23- dihydroxy- campest-4-en- 3-one FMNH2 Oxygen Hydrogen Ion Water FMN Campestanol 6a- hydroxycampestanol 6- Oxocampestanol 6- Deoxocathasterone Cathasterone Oxygen FMNH2 Teasterone Water Hydrogen Ion FMN Oxygen FMNH2 6- Deoxoteasterone Hydrogen Ion FMN Water 7-Oxateasterone 3- Dehydroteasterone 3-Dehydro-6- deoxo- teasterone Hydrogen Ion Water FMN Oxygen FMNH2 2- Deoxycastasterone 7- Oxatyphasterol Castasterone 6- Deoxocastasterone 6a- Hydroxycastasterone 26- Hydroxycastasterone Brassinolide 26- Hydroxybrassinolide Steroid Biosynthesis
CYP90B1 CYP90D1 CYP90A1 DET2 CYP90D1 DET2 CYP90B1 CYP90D1 CYP90B1 CYP90D1 CYP90D1 CYP85A2 CYP85A2 CYP90D2 CYP90D2 CYP90D1 CYP85A2 CYP90B1 CYP85A2 CYP85A2 At2g33210 At2g33210 CYP85A2 CYP85A2 CYP734A1 CYP85A2 CYP734A1 Campest 22a-Hyd O2 FMNH2 (2-22-D FMN H2O H+ HC4E3O H+ NADPH (22&α NADP 3-E-6-d O2 FMNH2 FMN H+ H2O 6-Deoxo C-4-e-3 45C H+ NADPH C3O NADP 2 2-2-d FMNH2 O2 H+ H2O FMN Campest 6a-hydr 6-Oxoca 6-Deoxo Catha O2 FMNH2 Teast H2O H+ FMN O2 FMNH2 6-Deoxo H+ FMN H2O 7-Oxate 3D 3-D-6-d H+ H2O FMN O2 FMNH2 Typhast 7-Oxaty Casta 6D 6a-Hydr 26-Hydr Brassin 26-Hydr PW122531 Mitochondrial Matrix Endoplasmic Reticulum Endoplasmic Reticulum
Endoplasmic Reticulum Endoplasmic Reticulum Mitochondria CYP90B1 CYP90D1 CYP90A1 DET2 CYP90D1 DET2 CYP90B1 CYP90D1 CYP90B1 CYP90D1 CYP90D1 CYP85A2 CYP85A2 CYP90D2 CYP90D2 CYP90D1 CYP85A2 CYP90B1 CYP85A2 CYP85A2 At2g33210 At2g33210 CYP85A2 CYP85A2 CYP734A1 CYP85A2 CYP734A1 Campest 22a-Hyd O2 FMNH2 (2-22-D FMN H2O H+ HC4E3O H+ NADPH (22&α NADP 3-E-6-d O2 FMNH2 FMN H+ H2O 6-Deoxo C-4-e-3 45C H+ NADPH C3O NADP 2 2-2-d FMNH2 O2 H+ H2O FMN Campest 6a-hydr 6-Oxoca 6-Deoxo Catha O2 FMNH2 Teast H2O H+ FMN O2 FMNH2 6-Deoxo H+ FMN H2O 7-Oxate 3D 3-D-6-d H+ H2O FMN O2 FMNH2 Typhast 7-Oxaty Casta 6D 6a-Hydr 26-Hydr Brassin 26-Hydr PW122531