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CHEBI:16015 ChEBI SubPathwayInteraction277 SubPathwayReaction SubPathway277Reaction 6022 PubChem-compound 937 ChemSpider CHEBI:16134 ChEBI HMDB01341 HMDB 1.0 1.0 439153 PubChem-compound SubPathwayInteraction271 SubPathway271Reaction SubPathwayReaction 1.0 SubPathwayInteraction272 SubPathwayReaction SubPathway272Reaction Tetrahydrofolic acid SubPathwayOutput SubPathwayInput 58-05-9 CAS SubPathwayInteraction270 SubPathway270Reaction SubPathwayReaction 10-FORMYL-THF BioCyc SubPathwayInteraction275 SubPathway275Reaction SubPathwayReaction Methylenetetrahydrofolate reductase SubPathwayInteraction276 SubPathway276Reaction SubPathwayReaction SubPathwayInteraction273 SubPathwayReaction SubPathway273Reaction 10-Formyltetrahydrofolate SubPathwayInteraction274 SubPathway274Reaction SubPathwayReaction SubPathwayInteraction268 SubPathway268Reaction SubPathwayReaction HMDB01354 HMDB L-Glutamic acid PW000056 PathWhiz SubPathwayInteraction269 SubPathway269Reaction SubPathwayReaction CHEBI:20506 ChEBI SubPathwayInteraction266 SubPathwayReaction SubPathway266Reaction 7444-29-3 CAS SubPathwayInteraction267 SubPathwayReaction SubPathway267Reaction HMDB00148 HMDB C19H21N7O6 Dihydrofolic acid 443.15533 CHEBI:17119 ChEBI C00440 KEGG Compound ATP BioCyc C00445 KEGG Compound 1.0 PW000052 PathWhiz HMDB00142 HMDB 5-METHYL-THF BioCyc 18714427 ChemSpider 2.0 Proton-coupled folate transporter SubPathwayInteraction265 SubPathway265Reaction SubPathwayReaction 390680 ChemSpider SMP00033 SMPDB PW000043 PathWhiz 5742 ChemSpider HMDB01562 HMDB CHEBI:16474 ChEBI PW000160 PathWhiz Carbon dioxide Formimidoyltransferase-cyclodeaminase HMDB59597 HMDB 439175 PubChem-compound P42898 UniProt 5-formyltetrahydrofolate cyclo-ligase SMP00046 SMPDB 1.0 SMP00044 SMPDB PW000157 PathWhiz METHYLENE-THF BioCyc 1010 ChemSpider HMDB00121 HMDB 6037 PubChem-compound C00101 KEGG Compound C6H9NO5 N-Formyl-L-glutamic acid 175.04807 SMP00050 SMPDB SMP00053 SMPDB 1.0 45479706 PubChem-compound HMDB00217 HMDB L-Glutamic acid SubPathwayOutput Tetrahydrofolyl-[Glu](n) C00234 KEGG Compound 5-FORMYL-THF BioCyc 1.0 Glutamate Metabolism SubPathway Histidine Metabolism SubPathway Methionine Metabolism SubPathway 122347 PubChem-compound Glycine and Serine Metabolism SubPathway Pyrimidine Metabolism SubPathway Purine Metabolism SubPathway Glycine and Serine Metabolism SubPathway Glycine and Serine Metabolism SubPathway HMDB00221 HMDB C00009 KEGG Compound C00008 KEGG Compound Q6UB35 UniProt C00006 KEGG Compound 5-Methyltetrahydrofolic acid SubPathwayOutput N-Formyl-L-glutamic acid CHEBI:18009 ChEBI O75891 UniProt C00001 KEGG Compound C00005 KEGG Compound CHEBI:18367 ChEBI SMILES OC(=O)CC[C@H](NC=O)C(O)=O C00004 KEGG Compound 135-16-0 CAS 1032 ChemSpider C00003 KEGG Compound NADP C00002 KEGG Compound 388320 ChemSpider Q96NT5 UniProt 1.0 HMDB01429 HMDB Phosphate 1.0 1.0 Tetrahydrofolyl-[Glu](n) C21H28N7O14P2 NAD 664.11694 Adenosine triphosphate C00011 KEGG Compound Formimidoyltransferase-cyclodeaminase C00014 KEGG Compound 1.0 Extracellular Space NAD(P) BioCyc 11267158 ChemSpider SMP00004 SMPDB H Hydrogen Ion 1.007825 HMDB01533 HMDB HMDB01534 HMDB C00143 KEGG Compound 56-65-5 CAS 53-84-9 CAS C00025 KEGG Compound C-1-tetrahydrofolate synthase, cytoplasmic 3432-99-3 CAS 8.0 CL:0000000 CELL TYPE ONTOLOGY C-1-tetrahydrofolate synthase, cytoplasmic C03541 KEGG Compound SMILES NC(=O)C1=CN(C=CC1)[C@@H]1O[C@H](CO[P@](O)(=O)O[P@](O)(=O)OC[C@H]2O[C@H]([C@H](O)[C@@H]2O)N2C=NC3=C(N)N=CN=C23)[C@@H](O)[C@H]1O 1.0 Adenosine diphosphate 98792 PubChem-compound L-methionyl-tRNA(Met) N-formylmethionyl-tRNA(fMet) 644350 PubChem-compound 1.0 SMILES NC1=NC=NC2=C1N=CN2[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O Folylpolyglutamate synthase, mitochondrial DIHYDROFOLATE BioCyc L-methionyl-tRNA(Met) HMDB00538 HMDB 1.0 Methylenetetrahydrofolate reductase 2.0 Cytosolic 10-formyltetrahydrofolate dehydrogenase 7732-18-5 CAS C00058 KEGG Compound NAD AMMONIA BioCyc SMILES N 56-86-0 CAS FORMATE BioCyc Adenosine diphosphate C10H16N5O13P3 Adenosine triphosphate 506.99576 C20H23N7O7 10-Formyltetrahydrofolate 473.1659 442163 PubChem-compound Q92820 UniProt C24H30N8O9 Tetrahydrofolyl-[Glu](2) 574.21356 C29H37N9O12 Tetrahydrofolyl-[Glu](n) 703.25616 2800-34-2 CAS 530 PubChem-compound 4033-27-6 CAS HMDB01846 HMDB HMDB01967 HMDB Ammonia Histidine Metabolism SubPathway Purine Metabolism SubPathway Methionine Metabolism SubPathway 1.0 Glycine and Serine Metabolism SubPathway Pyrimidine Metabolism SubPathway 962 PubChem-compound 5,10-Methylene-THF SubPathwayInput HMDB00972 HMDB 5800 ChemSpider C01045 KEGG Compound CO2 Carbon dioxide 43.98983 Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial C-1-tetrahydrofolate synthase, cytoplasmic C00080 KEGG Compound BiologicalState9 BiologicalState8 SMILES N[C@@H](CCC(O)=O)C(O)=O BiologicalState3 SMILES OC=O 14265-44-2 CAS 5,10-methenyl-thf BioCyc Methionyl-tRNA formyltransferase, mitochondrial Monofunctional C1-tetrahydrofolate synthase, mitochondrial 58-64-0 CAS C03479 KEGG Compound CHEBI:209153 ChEBI SMILES NC1=NC2=C(N(C=O)C(CNC3=CC=C(C=C3)C(=O)NC(CCC(O)=O)C(O)=O)CN2)C(=O)N1 FOLATE BioCyc SMILES CN1C(CNC2=CC=C(C=C2)C(=O)N[C@H](CCC(O)=O)C(O)=O)CNC2=C1C(=O)NC(N)=N2 10-Formyltetrahydrofolate SubPathwayInput 1.0 64-18-6 CAS 1.0 SMILES [H][C@@]12CN(C=[N+]1C1=C(NC2)NC(N)=NC1=O)C1=CC=C(C=C1)C(=O)N[C@@H](CCC(O)=O)C([O-])=O 1.0 SMILES NC1=NC(=O)C2=NC(CNC3=CC=C(C=C3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)=CN=C2N1 22833512 PubChem-compound N-formylmethionyl-tRNA(fMet) H2O Water 18.010565 Cytosolic 10-formyltetrahydrofolate dehydrogenase 1.0 NADH BioCyc 5-Formiminotetrahydrofolic acid SubPathwayOutput NAD BioCyc 5815 ChemSpider 559356 ChemSpider 5,10-Methenyltetrahydrofolic acid 1.0 Formimidoyltransferase-cyclodeaminase Formic acid SMILES NC1=NC2=C(N(C=N)C(CNC3=CC=C(C=C3)C(=O)NC(CCC(O)=O)C(O)=O)CN2)C(=O)N1 SMILES [H][C@@]12CN(CN1C1=C(NC2)N=C(N)NC1=O)C1=CC=C(C=C1)C(=O)NC(CCC(O)=O)C(O)=O 134-35-0 CAS NAD 1.0 30572 ChemSpider 17215925 ChemSpider 2.0 Folic acid 5-formyltetrahydrofolate cyclo-ligase Dihydrofolic acid SubPathwayInput SMILES O Methionyl-tRNA formyltransferase, mitochondrial H3N Ammonia 17.026548 1.0 2.0 Gamma-glutamyl hydrolase Dihydrofolate reductase Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial Water CHEBI:15846 ChEBI Folic acid ADP BioCyc 53-59-8 CAS Lysosome P00374 UniProt 1.0 CHEBI:48309 ChEBI 1038 PubChem-compound 143 PubChem-compound 278 ChemSpider C19H23N7O6 Tetrahydrofolic acid 445.171 2.0 274 ChemSpider Hydrogen Ion HMDB00902 HMDB CHEBI:16908 ChEBI 1.5.1.20 false 1.5.1.20 5,10-Methylene-THF + NADH → 5-Methyltetrahydrofolic acid + NAD LEFT_TO_RIGHT Reaction789 false 5-Formiminotetrahydrofolic acid ↔ 5,10-Methenyltetrahydrofolic acid + Ammonia REVERSIBLE O4P Phosphate 94.95342 Gamma-glutamyl hydrolase Dihydrofolate reductase Adenosine triphosphate TransportCatalysis229 ACTIVATION C21H30N7O17P3 NADPH 745.0911 6.3.3.2 false 6.3.3.2 Adenosine triphosphate + N5-Formyl-THF → 5,10-Methenyltetrahydrofolic acid + Adenosine diphosphate + Phosphate LEFT_TO_RIGHT 140 ChemSpider Reaction787 false 5,10-Methenyltetrahydrofolic acid + NADPH ↔ 5,10-Methylene-THF + NADP REVERSIBLE Reaction784 false L-Glutamic acid + N5-Formyl-THF → N-Formyl-L-glutamic acid + Tetrahydrofolic acid LEFT_TO_RIGHT C21H29N7O17P3 NADP 744.08325 Reaction785 false 10-Formyltetrahydrofolate + Hydrogen Ion ↔ 5,10-Methenyltetrahydrofolic acid + Water REVERSIBLE Mitochondrion THF BioCyc Reaction782 false Adenosine triphosphate + Formic acid + Tetrahydrofolic acid → 10-Formyltetrahydrofolate + Adenosine diphosphate + Phosphate LEFT_TO_RIGHT 1.5.1.6 false 1.5.1.6 10-Formyltetrahydrofolate + NADP + Water → Carbon dioxide + NADPH + Tetrahydrofolic acid LEFT_TO_RIGHT 1.5.1.3 false 1.5.1.3 NADP + Tetrahydrofolic acid ↔ Dihydrofolic acid + NADPH REVERSIBLE CHEBI:15641 ChEBI NADPH BioCyc 1.0 O95954 UniProt 217 ChemSpider SMILES NC1=NC(=O)C2=C(NC[C@H](CNC3=CC=C(C=C3)C(=O)NC(CCC(O)=O)C(O)=O)N2)N1 58-68-4 CAS Water SMILES [O-]P([O-])([O-])=O C20H21N7O6 5,10-Methenyltetrahydrofolic acid 455.15533 1.0 Dihydrofolate reductase CHEBI:15637 ChEBI 124-38-9 CAS CHEBI:15639 ChEBI GO:0005764 GENE ONTOLOGY C19H19N7O6 Folic acid 441.13968 Tetrahydrofolyl-[Glu](2) P13995 UniProt SMILES NC(=O)C1=C[N+](=CC=C1)[C@@H]1O[C@H](CO[P@](O)(=O)O[P@](O)(=O)OC[C@H]2O[C@H]([C@H](OP(O)(O)=O)[C@@H]2O)N2C=NC3=C(N)N=CN=C23)[C@@H](O)[C@H]1O 1.0 1.0 SMILES NC(=O)C1=CN(C=CC1)[C@H]1O[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2O[C@@H]([C@@H](OP(O)(O)=O)[C@H]2O)N2C=NC3=C(N)N=CN=C23)[C@H](O)[C@@H]1O C20H23N7O6 5,10-Methylene-THF 457.171 C20H24N8O6 5-Formiminotetrahydrofolic acid 472.1819 Q96DP5 UniProt GLT BioCyc 7664-41-7 CAS 53-57-6 CAS P11586 UniProt SMILES O=C=O 1.0 222 PubChem-compound Proton-coupled folate transporter Monofunctional C1-tetrahydrofolate synthase, mitochondrial C21H29N7O14P2 NADH 665.12476 1.0 5-FORMIMINO-THF BioCyc 5957 PubChem-compound 1681-96-5 CAS CHEBI:15633 ChEBI CHEBI:15636 ChEBI Folylpolyglutamate synthase, mitochondrial Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial 5,10-Methenyltetrahydrofolic acid 1.0 GO:0005739 GENE ONTOLOGY Monofunctional C1-tetrahydrofolate synthase, mitochondrial C10H15N5O10P2 Adenosine diphosphate 427.02942 GO:0005615 GENE ONTOLOGY GO:0005737 GENE ONTOLOGY 1.0 1.5.1.3 false 1.5.1.3 Dihydrofolic acid + NADP ↔ Folic acid + NADPH REVERSIBLE Transport24 false Folic acid (↔) Transport: Homo sapiens, Extracellular Space to Homo sapiens, Cell, Cytoplasm LEFT_TO_RIGHT 1.0 NADH CPD-8587 BioCyc SMILES NC(=O)C1=C[N+](=CC=C1)[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OC[C@H]2O[C@H]([C@H](O)[C@@H]2O)N2C=NC3=C2N=CN=C3N)[C@@H](O)[C@H]1O Transport22 false Tetrahydrofolic acid (→) Transport: Homo sapiens, Cell, Cytoplasm to Homo sapiens, Cell, Mitochondrion LEFT_TO_RIGHT 5682 ChemSpider Q05932 UniProt 1.0 1.0 21231977 ChemSpider 5675 ChemSpider P49914 UniProt 18971002 PubChem-compound Cytoplasm 5,10-Methylene-THF SubPathwayInput NADPH Cell Gamma-glutamyl hydrolase CHEBI:15422 ChEBI CHEBI:16635 ChEBI ReactionCatalysis330 ACTIVATION 388299 ChemSpider Hydrogen Ion CH2O2 Formic acid 46.005478 Tetrahydrofolyl-[Glu](2) CHEBI:28624 ChEBI 1.0 ReactionCatalysis328 ACTIVATION ReactionCatalysis329 ACTIVATION ReactionCatalysis326 ACTIVATION ReactionCatalysis324 ACTIVATION CHEBI:27650 ChEBI C5H9NO4 L-Glutamic acid 147.05316 ReactionCatalysis325 ACTIVATION C00504 KEGG Compound 33032 PubChem-compound ReactionCatalysis322 ACTIVATION ReactionCatalysis323 ACTIVATION 1.0 ReactionCatalysis320 ACTIVATION Water ReactionCatalysis321 ACTIVATION CHEBI:16526 ChEBI SMILES NC1=NC2=C(N=C(CNC3=CC=C(C=C3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)CN2)C(=O)N1 HMDB03470 HMDB HMDB01056 HMDB CHEBI:16761 ChEBI C20H23N7O7 N5-Formyl-THF 473.1659 C20H25N7O6 5-Methyltetrahydrofolic acid 459.18665 ReactionCatalysis319 ACTIVATION ReactionCatalysis317 ACTIVATION ReactionCatalysis318 ACTIVATION ReactionCatalysis315 ACTIVATION ReactionCatalysis316 ACTIVATION NADH ReactionCatalysis313 ACTIVATION ReactionCatalysis314 ACTIVATION PW000024 PathWhiz 9606 TAXONOMY 1.0 1.0 HMDB00051 HMDB 1.0 5893 PubChem-compound HMDB02111 HMDB Formic acid 439234 PubChem-compound 6.3.2.17 false 6.3.2.17 Adenosine triphosphate + L-Glutamic acid + Tetrahydrofolic acid → Adenosine diphosphate + Phosphate + Tetrahydrofolyl-[Glu](2) LEFT_TO_RIGHT CHEBI:30751 ChEBI SMILES NC1=NC(=O)C2=C(NCC(CN(C=O)C3=CC=C(C=C3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)N2)N1 2.0 CHEBI:27470 ChEBI Methylenetetrahydrofolate reductase 2.1.2.9 false 2.1.2.9 10-Formyltetrahydrofolate + L-methionyl-tRNA(Met) → N-formylmethionyl-tRNA(fMet) + Tetrahydrofolic acid LEFT_TO_RIGHT Reaction794 false 5,10-Methenyltetrahydrofolic acid + NADH ↔ 5,10-Methylene-THF + NAD REVERSIBLE 6.3.2.17 false 6.3.2.17 Adenosine triphosphate + L-Glutamic acid + Tetrahydrofolyl-[Glu](2) → Adenosine diphosphate + Phosphate + Tetrahydrofolyl-[Glu](n) LEFT_TO_RIGHT 3.4.19.9 false 3.4.19.9 Tetrahydrofolyl-[Glu](n) + Water → L-Glutamic acid + Tetrahydrofolyl-[Glu](2) LEFT_TO_RIGHT 1.0 CHEBI:15377 ChEBI CHEBI:15378 ChEBI SMILES [H+] SMILES NC1=NC=NC2=C1N=CN2[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O 5886 PubChem-compound SMILES NC1=NC2=C(NC(CNC3=CC=C(C=C3)C(=O)NC(CCC(=O)NC(CCC(O)=O)C(O)=O)C(O)=O)CN2)C(=O)N1 1.0 Phosphate SMILES NC1=NC(=O)C2=C(NCC(CNC3=CC=C(C=C3)C(=O)NC(CCC(=O)NC(CCC(=O)NC(CCC(O)=O)C(O)=O)C(O)=O)C(O)=O)N2)N1 HMDB01396 HMDB 1.0 SMP00072 SMPDB 280 PubChem-compound HMDB01487 HMDB C00415 KEGG Compound Folate Metabolism PW000003 PathWhiz 89228 ChemSpider 388371 ChemSpider 1.0 388496 ChemSpider 2311-81-1 CAS 439376 PubChem-compound 1061 PubChem-compound CPD-600 BioCyc 109092 ChemSpider HMDB06826 HMDB HMDB06825 HMDB C00664 KEGG Compound Tetrahydrofolic acid SubPathwayInput 13781857 PubChem-compound N5-Formyl-THF 1.0 59-30-3 CAS Proton-coupled folate transporter Dihydrofolate reductase Dihydrofolate reductase C-1- tetrahydrofolate synthase, cytoplasmic Cytosolic 10- formyltetrahydrofolate dehydrogenase Formimidoyltransferase- cyclodeaminase C-1- tetrahydrofolate synthase, cytoplasmic 5- formyltetrahydrofolate cyclo-ligase C-1- tetrahydrofolate synthase, cytoplasmic Methylenetetrahydrofolate reductase Formimidoyltransferase- cyclodeaminase Folylpolyglutamate synthase, mitochondrial Folylpolyglutamate synthase, mitochondrial Gamma-glutamyl hydrolase Monofunctional C1- tetrahydrofolate synthase, mitochondrial Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial Methionyl-tRNA formyltransferase, mitochondrial Tetrahydrofolic acid Dihydrofolic acid L-Glutamic acid 5-Formiminotetrahydrofolic acid 5,10-Methylene-THF 5-Methyltetrahydrofolic acid 10-Formyltetrahydrofolate Tetrahydrofolic acid 5,10-Methylene-THF Folic acid Folic acid NADP NADPH NADP NADPH ATP Formic acid ADP P i NADP H 2 O CO 2 NADPH N5-Formyl-THF N-Formyl-L- glutamic acid H + 5,10-Methenyltetrahydrofolic acid H 2 O ATP ADP P i NADPH NADP NADH NAD NH 3 L-Glutamic acid ATP Tetrahydrofolyl-[Glu](2) ADP P i ATP L-Glutamic acid Tetrahydrofolyl-[Glu](n) ADP P i H 2 O L-Glutamic acid ATP Formic acid ADP 10-Formyltetrahydrofolate P i H + 5,10-Methenyltetrahydrofolic acid H 2 O NADH NAD Pyridoxal 5'-phosphate Magnesium FAD Pyridoxal 5'-phosphate Calcium Calcium Magnesium Magnesium L- methionyl- tRNA(Met) N- formylmethionyl- tRNA(fMet) Histidine Metabolism Purine Metabolism Methionine Metabolism Glycine and Serine Metabolism Pyrimidine Metabolism Glutamate Metabolism Histidine Metabolism Glycine and Serine Metabolism Pyrimidine Metabolism Methionine Metabolism Purine Metabolism Glycine and Serine Metabolism Glycine and Serine Metabolism Mitochondria Lysosome Extracellular Space Intracellular Space Mitochondrial matrix
CHEBI:16015 ChEBI SubPathwayInteraction277 SubPathwayReaction SubPathway277Reaction 6022 PubChem-compound 937 ChemSpider CHEBI:16134 ChEBI HMDB01341 HMDB 1.0 1.0 439153 PubChem-compound SubPathwayInteraction271 SubPathway271Reaction SubPathwayReaction 1.0 SubPathwayInteraction272 SubPathwayReaction SubPathway272Reaction Tetrahydrofolic acid SubPathwayOutput SubPathwayInput 58-05-9 CAS SubPathwayInteraction270 SubPathway270Reaction SubPathwayReaction 10-FORMYL-THF BioCyc SubPathwayInteraction275 SubPathway275Reaction SubPathwayReaction Methylenetetrahydrofolate reductase SubPathwayInteraction276 SubPathway276Reaction SubPathwayReaction SubPathwayInteraction273 SubPathwayReaction SubPathway273Reaction 10-Formyltetrahydrofolate SubPathwayInteraction274 SubPathway274Reaction SubPathwayReaction SubPathwayInteraction268 SubPathway268Reaction SubPathwayReaction HMDB01354 HMDB L-Glutamic acid PW000056 PathWhiz SubPathwayInteraction269 SubPathway269Reaction SubPathwayReaction CHEBI:20506 ChEBI SubPathwayInteraction266 SubPathwayReaction SubPathway266Reaction 7444-29-3 CAS SubPathwayInteraction267 SubPathwayReaction SubPathway267Reaction HMDB00148 HMDB C19H21N7O6 Dihydrofolic acid 443.15533 CHEBI:17119 ChEBI C00440 KEGG Compound ATP BioCyc C00445 KEGG Compound 1.0 PW000052 PathWhiz HMDB00142 HMDB 5-METHYL-THF BioCyc 18714427 ChemSpider 2.0 Proton-coupled folate transporter SubPathwayInteraction265 SubPathway265Reaction SubPathwayReaction 390680 ChemSpider http://identifiers.org/smpdb/SMP00033 SMPDB PW000043 PathWhiz 5742 ChemSpider HMDB01562 HMDB CHEBI:16474 ChEBI PW000160 PathWhiz Carbon dioxide Formimidoyltransferase-cyclodeaminase HMDB59597 HMDB 439175 PubChem-compound P42898 UniProt 5-formyltetrahydrofolate cyclo-ligase http://identifiers.org/smpdb/SMP00046 SMPDB 1.0 http://identifiers.org/smpdb/SMP00044 SMPDB PW000157 PathWhiz METHYLENE-THF BioCyc 1010 ChemSpider HMDB00121 HMDB 6037 PubChem-compound C00101 KEGG Compound C6H9NO5 N-Formyl-L-glutamic acid 175.04807 http://identifiers.org/smpdb/SMP00050 SMPDB http://identifiers.org/smpdb/SMP00053 SMPDB 1.0 45479706 PubChem-compound HMDB00217 HMDB L-Glutamic acid SubPathwayOutput Tetrahydrofolyl-[Glu](n) C00234 KEGG Compound 5-FORMYL-THF BioCyc 1.0 Glutamate Metabolism SubPathway Histidine Metabolism SubPathway Methionine Metabolism SubPathway 122347 PubChem-compound Glycine and Serine Metabolism SubPathway Pyrimidine Metabolism SubPathway Purine Metabolism SubPathway Glycine and Serine Metabolism SubPathway Glycine and Serine Metabolism SubPathway HMDB00221 HMDB C00009 KEGG Compound C00008 KEGG Compound Q6UB35 UniProt C00006 KEGG Compound 5-Methyltetrahydrofolic acid SubPathwayOutput N-Formyl-L-glutamic acid CHEBI:18009 ChEBI O75891 UniProt C00001 KEGG Compound C00005 KEGG Compound CHEBI:18367 ChEBI SMILES OC(=O)CC[C@H](NC=O)C(O)=O C00004 KEGG Compound 135-16-0 CAS 1032 ChemSpider C00003 KEGG Compound NADP C00002 KEGG Compound 388320 ChemSpider Q96NT5 UniProt 1.0 HMDB01429 HMDB Phosphate 1.0 1.0 Tetrahydrofolyl-[Glu](n) C21H28N7O14P2 NAD 664.11694 Adenosine triphosphate C00011 KEGG Compound Formimidoyltransferase-cyclodeaminase C00014 KEGG Compound 1.0 Extracellular Space NAD(P) BioCyc 11267158 ChemSpider http://identifiers.org/smpdb/SMP00004 SMPDB H Hydrogen Ion 1.007825 HMDB01533 HMDB HMDB01534 HMDB C00143 KEGG Compound 56-65-5 CAS 53-84-9 CAS C00025 KEGG Compound C-1-tetrahydrofolate synthase, cytoplasmic 3432-99-3 CAS 8.0 CL:0000000 CELL TYPE ONTOLOGY C-1-tetrahydrofolate synthase, cytoplasmic C03541 KEGG Compound SMILES NC(=O)C1=CN(C=CC1)[C@@H]1O[C@H](CO[P@](O)(=O)O[P@](O)(=O)OC[C@H]2O[C@H]([C@H](O)[C@@H]2O)N2C=NC3=C(N)N=CN=C23)[C@@H](O)[C@H]1O 1.0 Adenosine diphosphate 98792 PubChem-compound L-methionyl-tRNA(Met) N-formylmethionyl-tRNA(fMet) 644350 PubChem-compound 1.0 SMILES NC1=NC=NC2=C1N=CN2[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O Folylpolyglutamate synthase, mitochondrial DIHYDROFOLATE BioCyc L-methionyl-tRNA(Met) HMDB00538 HMDB 1.0 Methylenetetrahydrofolate reductase 2.0 Cytosolic 10-formyltetrahydrofolate dehydrogenase 7732-18-5 CAS C00058 KEGG Compound NAD AMMONIA BioCyc SMILES N 56-86-0 CAS FORMATE BioCyc Adenosine diphosphate C10H16N5O13P3 Adenosine triphosphate 506.99576 C20H23N7O7 10-Formyltetrahydrofolate 473.1659 442163 PubChem-compound Q92820 UniProt C24H30N8O9 Tetrahydrofolyl-[Glu](2) 574.21356 C29H37N9O12 Tetrahydrofolyl-[Glu](n) 703.25616 2800-34-2 CAS 530 PubChem-compound 4033-27-6 CAS HMDB01846 HMDB HMDB01967 HMDB Ammonia Histidine Metabolism SubPathway Purine Metabolism SubPathway Methionine Metabolism SubPathway 1.0 Glycine and Serine Metabolism SubPathway Pyrimidine Metabolism SubPathway 962 PubChem-compound 5,10-Methylene-THF SubPathwayInput HMDB00972 HMDB 5800 ChemSpider C01045 KEGG Compound CO2 Carbon dioxide 43.98983 Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial C-1-tetrahydrofolate synthase, cytoplasmic C00080 KEGG Compound BiologicalState9 Homo sapiens, Cell, Lysosome BiologicalState8 Homo sapiens, Cell, Cytoplasm SMILES N[C@@H](CCC(O)=O)C(O)=O BiologicalState3 Homo sapiens, Cell, Mitochondrion SMILES OC=O 14265-44-2 CAS 5,10-methenyl-thf BioCyc Methionyl-tRNA formyltransferase, mitochondrial Monofunctional C1-tetrahydrofolate synthase, mitochondrial 58-64-0 CAS C03479 KEGG Compound CHEBI:209153 ChEBI SMILES NC1=NC2=C(N(C=O)C(CNC3=CC=C(C=C3)C(=O)NC(CCC(O)=O)C(O)=O)CN2)C(=O)N1 FOLATE BioCyc SMILES CN1C(CNC2=CC=C(C=C2)C(=O)N[C@H](CCC(O)=O)C(O)=O)CNC2=C1C(=O)NC(N)=N2 10-Formyltetrahydrofolate SubPathwayInput 1.0 64-18-6 CAS 1.0 SMILES [H][C@@]12CN(C=[N+]1C1=C(NC2)NC(N)=NC1=O)C1=CC=C(C=C1)C(=O)N[C@@H](CCC(O)=O)C([O-])=O 1.0 SMILES NC1=NC(=O)C2=NC(CNC3=CC=C(C=C3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)=CN=C2N1 22833512 PubChem-compound N-formylmethionyl-tRNA(fMet) H2O Water 18.010565 Cytosolic 10-formyltetrahydrofolate dehydrogenase 1.0 NADH BioCyc 5-Formiminotetrahydrofolic acid SubPathwayOutput NAD BioCyc 5815 ChemSpider 559356 ChemSpider 5,10-Methenyltetrahydrofolic acid 1.0 Formimidoyltransferase-cyclodeaminase Formic acid SMILES NC1=NC2=C(N(C=N)C(CNC3=CC=C(C=C3)C(=O)NC(CCC(O)=O)C(O)=O)CN2)C(=O)N1 SMILES [H][C@@]12CN(CN1C1=C(NC2)N=C(N)NC1=O)C1=CC=C(C=C1)C(=O)NC(CCC(O)=O)C(O)=O 134-35-0 CAS NAD 1.0 30572 ChemSpider 17215925 ChemSpider 2.0 Folic acid 5-formyltetrahydrofolate cyclo-ligase Dihydrofolic acid SubPathwayInput SMILES O Methionyl-tRNA formyltransferase, mitochondrial H3N Ammonia 17.026548 1.0 2.0 Gamma-glutamyl hydrolase Dihydrofolate reductase Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial Water CHEBI:15846 ChEBI Folic acid ADP BioCyc 53-59-8 CAS Lysosome P00374 UniProt 1.0 CHEBI:48309 ChEBI 1038 PubChem-compound 143 PubChem-compound 278 ChemSpider C19H23N7O6 Tetrahydrofolic acid 445.171 2.0 274 ChemSpider Hydrogen Ion HMDB00902 HMDB CHEBI:16908 ChEBI 1.5.1.20 false 1.5.1.20 5,10-Methylene-THF + NADH → 5-Methyltetrahydrofolic acid + NAD LEFT_TO_RIGHT Reaction789 false 5-Formiminotetrahydrofolic acid ↔ 5,10-Methenyltetrahydrofolic acid + Ammonia REVERSIBLE O4P Phosphate 94.95342 Gamma-glutamyl hydrolase Dihydrofolate reductase Adenosine triphosphate TransportCatalysis229 ACTIVATION C21H30N7O17P3 NADPH 745.0911 6.3.3.2 false 6.3.3.2 Adenosine triphosphate + N5-Formyl-THF → 5,10-Methenyltetrahydrofolic acid + Adenosine diphosphate + Phosphate LEFT_TO_RIGHT 140 ChemSpider Reaction787 false 5,10-Methenyltetrahydrofolic acid + NADPH ↔ 5,10-Methylene-THF + NADP REVERSIBLE Reaction784 false L-Glutamic acid + N5-Formyl-THF → N-Formyl-L-glutamic acid + Tetrahydrofolic acid LEFT_TO_RIGHT C21H29N7O17P3 NADP 744.08325 Reaction785 false 10-Formyltetrahydrofolate + Hydrogen Ion ↔ 5,10-Methenyltetrahydrofolic acid + Water REVERSIBLE Mitochondrion THF BioCyc Reaction782 false Adenosine triphosphate + Formic acid + Tetrahydrofolic acid → 10-Formyltetrahydrofolate + Adenosine diphosphate + Phosphate LEFT_TO_RIGHT 1.5.1.6 false 1.5.1.6 10-Formyltetrahydrofolate + NADP + Water → Carbon dioxide + NADPH + Tetrahydrofolic acid LEFT_TO_RIGHT 1.5.1.3 false 1.5.1.3 NADP + Tetrahydrofolic acid ↔ Dihydrofolic acid + NADPH REVERSIBLE CHEBI:15641 ChEBI NADPH BioCyc 1.0 O95954 UniProt 217 ChemSpider SMILES NC1=NC(=O)C2=C(NC[C@H](CNC3=CC=C(C=C3)C(=O)NC(CCC(O)=O)C(O)=O)N2)N1 58-68-4 CAS Water SMILES [O-]P([O-])([O-])=O C20H21N7O6 5,10-Methenyltetrahydrofolic acid 455.15533 1.0 Dihydrofolate reductase CHEBI:15637 ChEBI 124-38-9 CAS CHEBI:15639 ChEBI GO:0005764 GENE ONTOLOGY C19H19N7O6 Folic acid 441.13968 Tetrahydrofolyl-[Glu](2) P13995 UniProt SMILES NC(=O)C1=C[N+](=CC=C1)[C@@H]1O[C@H](CO[P@](O)(=O)O[P@](O)(=O)OC[C@H]2O[C@H]([C@H](OP(O)(O)=O)[C@@H]2O)N2C=NC3=C(N)N=CN=C23)[C@@H](O)[C@H]1O 1.0 1.0 SMILES NC(=O)C1=CN(C=CC1)[C@H]1O[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2O[C@@H]([C@@H](OP(O)(O)=O)[C@H]2O)N2C=NC3=C(N)N=CN=C23)[C@H](O)[C@@H]1O C20H23N7O6 5,10-Methylene-THF 457.171 C20H24N8O6 5-Formiminotetrahydrofolic acid 472.1819 Q96DP5 UniProt GLT BioCyc 7664-41-7 CAS 53-57-6 CAS P11586 UniProt SMILES O=C=O 1.0 222 PubChem-compound Proton-coupled folate transporter Monofunctional C1-tetrahydrofolate synthase, mitochondrial C21H29N7O14P2 NADH 665.12476 1.0 5-FORMIMINO-THF BioCyc 5957 PubChem-compound 1681-96-5 CAS CHEBI:15633 ChEBI CHEBI:15636 ChEBI Folylpolyglutamate synthase, mitochondrial Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial 5,10-Methenyltetrahydrofolic acid 1.0 GO:0005739 GENE ONTOLOGY Monofunctional C1-tetrahydrofolate synthase, mitochondrial C10H15N5O10P2 Adenosine diphosphate 427.02942 GO:0005615 GENE ONTOLOGY GO:0005737 GENE ONTOLOGY 1.0 1.5.1.3 false 1.5.1.3 Dihydrofolic acid + NADP ↔ Folic acid + NADPH REVERSIBLE Transport24 false Folic acid (↔) Transport: Homo sapiens, Extracellular Space to Homo sapiens, Cell, Cytoplasm LEFT_TO_RIGHT 1.0 NADH CPD-8587 BioCyc SMILES NC(=O)C1=C[N+](=CC=C1)[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OC[C@H]2O[C@H]([C@H](O)[C@@H]2O)N2C=NC3=C2N=CN=C3N)[C@@H](O)[C@H]1O Transport22 false Tetrahydrofolic acid (→) Transport: Homo sapiens, Cell, Cytoplasm to Homo sapiens, Cell, Mitochondrion LEFT_TO_RIGHT 5682 ChemSpider Q05932 UniProt 1.0 1.0 21231977 ChemSpider 5675 ChemSpider P49914 UniProt 18971002 PubChem-compound Cytoplasm 5,10-Methylene-THF SubPathwayInput NADPH Cell Gamma-glutamyl hydrolase CHEBI:15422 ChEBI CHEBI:16635 ChEBI ReactionCatalysis330 ACTIVATION 388299 ChemSpider Hydrogen Ion CH2O2 Formic acid 46.005478 Tetrahydrofolyl-[Glu](2) CHEBI:28624 ChEBI 1.0 ReactionCatalysis328 ACTIVATION ReactionCatalysis329 ACTIVATION ReactionCatalysis326 ACTIVATION ReactionCatalysis324 ACTIVATION CHEBI:27650 ChEBI C5H9NO4 L-Glutamic acid 147.05316 ReactionCatalysis325 ACTIVATION C00504 KEGG Compound 33032 PubChem-compound ReactionCatalysis322 ACTIVATION ReactionCatalysis323 ACTIVATION 1.0 ReactionCatalysis320 ACTIVATION Water ReactionCatalysis321 ACTIVATION CHEBI:16526 ChEBI SMILES NC1=NC2=C(N=C(CNC3=CC=C(C=C3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)CN2)C(=O)N1 HMDB03470 HMDB HMDB01056 HMDB CHEBI:16761 ChEBI C20H23N7O7 N5-Formyl-THF 473.1659 C20H25N7O6 5-Methyltetrahydrofolic acid 459.18665 ReactionCatalysis319 ACTIVATION ReactionCatalysis317 ACTIVATION ReactionCatalysis318 ACTIVATION ReactionCatalysis315 ACTIVATION ReactionCatalysis316 ACTIVATION NADH ReactionCatalysis313 ACTIVATION ReactionCatalysis314 ACTIVATION PW000024 PathWhiz 9606 TAXONOMY 1.0 1.0 HMDB00051 HMDB 1.0 5893 PubChem-compound HMDB02111 HMDB Formic acid 439234 PubChem-compound 6.3.2.17 false 6.3.2.17 Adenosine triphosphate + L-Glutamic acid + Tetrahydrofolic acid → Adenosine diphosphate + Phosphate + Tetrahydrofolyl-[Glu](2) LEFT_TO_RIGHT CHEBI:30751 ChEBI SMILES NC1=NC(=O)C2=C(NCC(CN(C=O)C3=CC=C(C=C3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)N2)N1 2.0 CHEBI:27470 ChEBI Methylenetetrahydrofolate reductase 2.1.2.9 false 2.1.2.9 10-Formyltetrahydrofolate + L-methionyl-tRNA(Met) → N-formylmethionyl-tRNA(fMet) + Tetrahydrofolic acid LEFT_TO_RIGHT Reaction794 false 5,10-Methenyltetrahydrofolic acid + NADH ↔ 5,10-Methylene-THF + NAD REVERSIBLE 6.3.2.17 false 6.3.2.17 Adenosine triphosphate + L-Glutamic acid + Tetrahydrofolyl-[Glu](2) → Adenosine diphosphate + Phosphate + Tetrahydrofolyl-[Glu](n) LEFT_TO_RIGHT 3.4.19.9 false 3.4.19.9 Tetrahydrofolyl-[Glu](n) + Water → L-Glutamic acid + Tetrahydrofolyl-[Glu](2) LEFT_TO_RIGHT 1.0 CHEBI:15377 ChEBI CHEBI:15378 ChEBI SMILES [H+] SMILES NC1=NC=NC2=C1N=CN2[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O 5886 PubChem-compound SMILES NC1=NC2=C(NC(CNC3=CC=C(C=C3)C(=O)NC(CCC(=O)NC(CCC(O)=O)C(O)=O)C(O)=O)CN2)C(=O)N1 1.0 Phosphate SMILES NC1=NC(=O)C2=C(NCC(CNC3=CC=C(C=C3)C(=O)NC(CCC(=O)NC(CCC(=O)NC(CCC(O)=O)C(O)=O)C(O)=O)C(O)=O)N2)N1 HMDB01396 HMDB 1.0 http://identifiers.org/smpdb/SMP00072 SMPDB 280 PubChem-compound HMDB01487 HMDB C00415 KEGG Compound Folate Metabolism PW000003 PathWhiz 89228 ChemSpider 388371 ChemSpider 1.0 388496 ChemSpider 2311-81-1 CAS 439376 PubChem-compound 1061 PubChem-compound CPD-600 BioCyc 109092 ChemSpider HMDB06826 HMDB HMDB06825 HMDB C00664 KEGG Compound Tetrahydrofolic acid SubPathwayInput 13781857 PubChem-compound N5-Formyl-THF 1.0 59-30-3 CAS