PathBank

Pathway Description

S-Adenosyl-L-Methionine Cycle

Escherichia coli

Category:

Metabolite Pathway

Sub-Category:

Metabolic

Created: 2015-10-13

Last Updated: 2025-05-08

The S-adenosyl-L-methionine cycle starts with S-adenosyl-L-methionine reacting with (a demethylated methyl donor ) dimethylglycine resulting in the release of a hydrogen ion, a betain (a methylated methyl donor) and a S-adenosyl-L-homocysteine. The s-adenosyl-L-homocysteine reacts with a water molecule through a S-adenosylhomocysteine nucleosidase resulting in the release of a adenine and a ribosyl-L-homocysteine. This compound in turn reacts with a s-ribosylhomocysteine lyase resulting in the release of a l-homocysteine and a autoinducer 2. The L-homocysteine reacts with a N5-methyl-tetrahydropteroyl tri-L-glutamate through a methionine synthase resulting in the release of a tetrahydropteroyl tri-L-glutamate and a methione. The methionine in turn reacts with a water molecule and ATP molecule through a methionine adenosyltransferase resulting in the release of a diphosphate, a phosphate and a s-adenosyl-L-methionine.

References

S-Adenosyl-L-Methionine Cycle References

Halliday NM, Hardie KR, Williams P, Winzer K, Barrett DA: Quantitative liquid chromatography-tandem mass spectrometry profiling of activated methyl cycle metabolites involved in LuxS-dependent quorum sensing in Escherichia coli. Anal Biochem. 2010 Aug;403(1-2):20-9. doi: 10.1016/j.ab.2010.04.021. Epub 2010 Apr 22.

Pubmed: 20417170

Cornell KA, Riscoe MK: Cloning and expression of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase: identification of the pfs gene product. Biochim Biophys Acta. 1998 Mar 4;1396(1):8-14. doi: 10.1016/s0167-4781(97)00169-3.

Pubmed: 9524204

Cornell KA, Swarts WE, Barry RD, Riscoe MK: Characterization of recombinant Eschericha coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase: analysis of enzymatic activity and substrate specificity. Biochem Biophys Res Commun. 1996 Nov 21;228(3):724-32. doi: 10.1006/bbrc.1996.1723.

Pubmed: 8941345

Wurgler SM, Richardson CC: Structure and regulation of the gene for dGTP triphosphohydrolase from Escherichia coli. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2740-4. doi: 10.1073/pnas.87.7.2740.

Pubmed: 2157212

Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T, et al.: Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. DNA Res. 1997 Apr 28;4(2):91-113. doi: 10.1093/dnares/4.2.91.

Pubmed: 9205837

Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.

Pubmed: 9278503

Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. doi: 10.1038/msb4100049. Epub 2006 Feb 21.

Pubmed: 16738553

Old IG, Margarita D, Glass RE, Saint Girons I: Nucleotide sequence of the metH gene of Escherichia coli K-12 and comparison with that of Salmonella typhimurium LT2. Gene. 1990 Mar 1;87(1):15-21. doi: 10.1016/0378-1119(90)90490-i.

Pubmed: 2185137

Banerjee RV, Johnston NL, Sobeski JK, Datta P, Matthews RG: Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain. J Biol Chem. 1989 Aug 15;264(23):13888-95.

Pubmed: 2668277

Drummond JT, Loo RR, Matthews RG: Electrospray mass spectrometric analysis of the domains of a large enzyme: observation of the occupied cobalamin-binding domain and redefinition of the carboxyl terminus of methionine synthase. Biochemistry. 1993 Sep 14;32(36):9282-9. doi: 10.1021/bi00087a004.

Pubmed: 8369296

Markham GD, DeParasis J, Gatmaitan J: The sequence of metK, the structural gene for S-adenosylmethionine synthetase in Escherichia coli. J Biol Chem. 1984 Dec 10;259(23):14505-7.

Pubmed: 6094561

Satishchandran C, Taylor JC, Markham GD: Isozymes of S-adenosylmethionine synthetase are encoded by tandemly duplicated genes in Escherichia coli. Mol Microbiol. 1993 Aug;9(4):835-46. doi: 10.1111/j.1365-2958.1993.tb01742.x.

Pubmed: 8231813

This pathway was propagated using PathWhiz - Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.

Propagated from SMP0002092

	S-ribosyl-L-homocysteine
	5-Methyltetrahydrofolic acid
	Adenine
	Adenosine triphosphate
	autoinducer 2
	Dimethylglycine
	Homocysteine
	Hydrogen Ion
	L-Methionine
	Phosphate
	Pyrophosphate
	S-Adenosylhomocysteine
	S-Adenosylmethionine
	Tetrahydrofolic acid
	Water
	βine

	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
	Methionine synthase
	S-adenosylmethionine synthase
	S-ribosylhomocysteine lyase

		S-ribosyl-L-homocysteine
		5-Methyltetrahydrofolic acid
		Adenine
		Adenosine triphosphate
		autoinducer 2
		Dimethylglycine
		Homocysteine
		Hydrogen Ion
		L-Methionine
		Phosphate
		Pyrophosphate
		S-Adenosylhomocysteine
		S-Adenosylmethionine
		Tetrahydrofolic acid
		Water
		βine

		5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
		Methionine synthase
		S-adenosylmethionine synthase
		S-ribosylhomocysteine lyase

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S-Adenosyl-L-Methionine Cycle

S-Adenosyl-L-Methionine Cycle References