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Pathway Description

Operon: Folylpolyglutamate Synthase

Escherichia coli

Category:

Metabolite Pathway

Sub-Category:

Signaling

Created: 2015-10-30

Last Updated: 2019-09-30

The folC-dedD operon in E. coli contains two genes that are involved in various processes in the cell. There are currently no known activators or inhibitors of the operon. The first gene, folC, encodes a bifunctional dihydrofolate synthase and folylpolyglutamate synthase protein. This catalyzes the conversion of 7,8-dihydrofolate fro glutamate and d7,8-dihydropteroate, as well as the formation of folylpolyglutamates. These are further used in cofactor biosynthesis by the cell. The second gene, dedD, encodes a cell division protein. It is non-essential for the cell, and may be used during cell constriction during division, making the process more efficient.

References

Operon: Folylpolyglutamate Synthase References

Bermingham A, Derrick JP: The folic acid biosynthesis pathway in bacteria: evaluation of potential for antibacterial drug discovery. Bioessays. 2002 Jul;24(7):637-48. doi: 10.1002/bies.10114.

Pubmed: 12111724

Bognar AL, Osborne C, Shane B, Singer SC, Ferone R: Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product. J Biol Chem. 1985 May 10;260(9):5625-30.

Pubmed: 2985605

Bognar AL, Osborne C, Shane B: Primary structure of the Escherichia coli folC gene and its folylpolyglutamate synthetase-dihydrofolate synthetase product and regulation of expression by an upstream gene. J Biol Chem. 1987 Sep 5;262(25):12337-43.

Pubmed: 3040739

Kimlova LJ, Pyne C, Keshavjee K, Huy J, Beebakhee G, Bognar AL: Mutagenesis of the folC gene encoding folylpolyglutamate synthetase-dihydrofolate synthetase in Escherichia coli. Arch Biochem Biophys. 1991 Jan;284(1):9-16. doi: 10.1016/0003-9861(91)90254-g.

Pubmed: 1989505

Nonet ML, Marvel CC, Tolan DR: The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons. J Biol Chem. 1987 Sep 5;262(25):12209-17.

Pubmed: 3040734

Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T, et al.: Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. DNA Res. 1997 Apr 28;4(2):91-113. doi: 10.1093/dnares/4.2.91.

Pubmed: 9205837

Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.

Pubmed: 9278503

Highlighted elements will appear in red.

Highlight Compounds

	Magnesium
	Potassium

Highlight Proteins

	Bifunctional protein folC
	Cell division protein DedD

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