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Pathway Description
Secondary Metabolites: Threonine Biosynthesis from Aspartate
Pseudomonas aeruginosa
Category:
Metabolite Pathway
Sub-Category:
Metabolic
Created: 2019-08-12
Last Updated: 2019-08-16
The biosynthesis of threonine starts with L-aspartic acid being phosphorylated by an ATP driven Aspartate kinase resulting in an a release of an ADP and an L-aspartyl-4-phosphate. This compound interacts with a hydrogen ion through an NADPH driven aspartate semialdehyde dehydrogenase resulting in the release of a phosphate, an NADP and a L-aspartate-semialdehyde.The latter compound interacts with a hydrogen ion through a NADPH driven aspartate kinase / homoserine dehydrogenase resulting in the release of an NADP and a L-homoserine. L-homoserine is phosphorylated through an ATP driven homoserine kinase resulting in the release of an ADP, a hydrogen ion and a O-phosphohomoserine. The latter compound then interacts with a water molecule threonine synthase resulting in the release of a phosphate and an L-threonine.
References
Secondary Metabolites: Threonine Biosynthesis from Aspartate References
Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. doi: 10.1038/35023079.
Pubmed: 10984043
Hoang TT, Williams S, Schweizer HP, Lam JS: Molecular genetic analysis of the region containing the essential Pseudomonas aeruginosa asd gene encoding aspartate-beta-semialdehyde dehydrogenase. Microbiology. 1997 Mar;143 ( Pt 3):899-907. doi: 10.1099/00221287-143-3-899.
Pubmed: 9084174
Moore RA, Bocik WE, Viola RE: Expression and purification of aspartate beta-semialdehyde dehydrogenase from infectious microorganisms. Protein Expr Purif. 2002 Jun;25(1):189-94. doi: 10.1006/prep.2002.1626.
Pubmed: 12071715
Clepet C, Borne F, Krishnapillai V, Baird C, Patte JC, Cami B: Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes. Mol Microbiol. 1992 Nov;6(21):3109-19. doi: 10.1111/j.1365-2958.1992.tb01768.x.
Pubmed: 1333566
Kaur N, Gautam A, Kumar S, Singh A, Singh N, Sharma S, Sharma R, Tewari R, Singh TP: Biochemical studies and crystal structure determination of dihydrodipicolinate synthase from Pseudomonas aeruginosa. Int J Biol Macromol. 2011 Jun 1;48(5):779-87. doi: 10.1016/j.ijbiomac.2011.03.002. Epub 2011 Mar 10.
Pubmed: 21396954
This pathway was propagated using PathWhiz -
Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.
Propagated from SMP0000992
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