PathBank

Pathway Description

O-Antigen Building Blocks Biosynthesis

Pseudomonas aeruginosa

Category:

Metabolite Pathway

Sub-Category:

Metabolic

Created: 2019-08-12

Last Updated: 2019-10-08

Lipopolysaccharide (LPS) is a major component of outer membrane which is consisted of lipid A-core (oligosaccharide) on both inner and outer region and O-antigen (known as distal repeating unit with four sugars: N-acetylglucosamine, glucose, rhamnose and galactose). O-antigen is part of three domains of LPS, which is attached to lipid A-core; however, O-antigen and lipid A-core are synthesized separately. In this pathway, synthesis of three of O-antigen sugars is demonstrated. UDP-α-D-galactose is converted to UDP-D-Galacto-1,4-furanose by facilitation of UDP-galactopyranose mutase. dTTP glucose-1-phosphate is derivatized to dTDP-rhamnose. Fructose-6-phosphate gains an amino group, incorporates an acetate moiety and then acquires a nucleoside diphosphate resulting in UDP-N-acetyl-D-glucosamine.

References

O-Antigen Building Blocks Biosynthesis References

Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. doi: 10.1038/35023079.

Pubmed: 10984043

Dong C, Major LL, Srikannathasan V, Errey JC, Giraud MF, Lam JS, Graninger M, Messner P, McNeil MR, Field RA, Whitfield C, Naismith JH: RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation. J Mol Biol. 2007 Jan 5;365(1):146-59. doi: 10.1016/j.jmb.2006.09.063. Epub 2006 Sep 29.

Pubmed: 17046787

This pathway was propagated using PathWhiz - Pon, A. et al. Pathways with PathWhiz (2015) Nucleic Acids Res. 43(Web Server issue): W552–W559.

Propagated from SMP0002101

Enter relative concentration values (without units). Elements will be highlighted in a color gradient where red = lowest concentration and green = highest concentration. For the best results, view the pathway in Black and White.

Visualize Compound Data

		Acetyl-CoA
		Coenzyme A
		D-glucosamine 6-phosphate
		diphosphate
		dTDP-4-dehydro-6-deoxy-D-glucose
		dTDP-4-dehydro-β-L-rhamnose
		dTDP-6-deoxy-L-mannose
		dTDP-D-glucose
		Glucosamine-1P
		Hydrogen Ion
		L-Glutamic acid
		L-Glutamine
		Magnesium
		N-Acetyl-glucosamine 1-phosphate
		NAD
		NADH
		NADP
		NADPH
		TDP-Rhamnose
		Thymidine 5'-triphosphate
		UDP-D-Galacto-1,4-furanose
		UDP-α-D-galactose
		Uridine diphosphate-N-acetylglucosamine
		Uridine triphosphate
		Water
		α-D-Glucose 1-phosphate
		β-D-fructofuranose 6-phosphate
		β-D-Glucose 6-phosphate

Visualize Protein Data

		Bifunctional protein GlmU
		dTDP-4-dehydrorhamnose 3,5-epimerase
		dTDP-4-dehydrorhamnose reductase
		dTDP-glucose 4,6-dehydratase
		Glucose-6-phosphate isomerase
		Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
		Phosphoglucosamine mutase
		UTP--glucose-1-phosphate uridylyltransferase