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PKA complex Beta-2 adrenergic receptor Adenylate cyclase type 9 PKA complex PKA complex PKA complex Myosin light chain kinase, smooth muscle Actin, cytoplasmic 1 Myosin LC-P Myosin light chain 3 Myosin light chain kinase, smooth muscle Calmodulin-1 Serine/threonine- protein phosphatase PP1-beta catalytic subunit Calmodulin-1 PKA complex PKA complex cAMP Epinephrine Ca+ Ca+ ATP Magnesium Magnesium Calcium Manganese Ca+ Contraction Relaxation Other Intracellular Signaling Pathways Voltage- dependent P/Q-type calcium channel subunit alpha-1A Voltage- dependent L-type calcium channel subunit beta-1 Voltage- dependent calcium channel subunit alpha-2/delta-2 Epinephrine activates beta-2-adrenergic receptors in the smooth muscle of the respiratory airways Activation of beta-2adrenergic recepotrs activates the Gs signaling cascade leading to increased levels of cAMP and activation of protein kinase A. PKA inactivates myosin light chain kinase by phosphorylating it Myosin light chain kinase phosphorylates myosin light chain, whereas myosin light chain phosphatase dephosphorylates myosin LC-P Accumulation of myosin LC leads to increased relaxation of the airway smooth muscles Myosin LC-P interacts with actin to produce smooth muscle contraction. Since there are low levels of myosin LC-P, contraction is inhibited PKA may also phosphorylate Gq-coupled receptors leading to a cascade of intracellular signals which reduce intracellular Ca2+ Caclium usually binds to calmodulin to activate myosin light chain kinase. Since Ca2+ levles are low, activation of myosin light chain kinase is reduced. Epinephrine can be administered via respiratory inhalation Actin Myosin Actin unbinds from myosin allowing muscle relaxation to occur
PRKAR2B ADRB2 ADCY9 PRKACB PRKAR1B PRKAR2A MYLK ACTB MYL3 MYL3 MYLK CALM1 PPP1CB CALM1 PRKACG PRKAR1A cAMP Epinephrine Calcium Calcium Adenosine triphosphate Calcium Contraction Relaxation Other Intracellular Signaling Pathways CACNA1A CACNB1 CACNA2D2
PRKAR2B ADRB2 ADCY9 PRKACB PRKAR1B PRKAR2A MYLK ACTB MYL3 MYL3 MYLK CALM1 PPP1CB CALM1 PRKACG PRKAR1A cAMP Eppy Ca+ Ca+ ATP Mg2+ Mg2+ Ca2+ Mn2+ Ca+ Contr Relax O I S P CACNA1A CACNB1 CACNA2D2 Epinephrine activates beta-2-adrenergic receptors in the smooth muscle of the respiratory airways Activation of beta-2adrenergic recepotrs activates the Gs signaling cascade leading to increased levels of cAMP and activation of protein kinase A. PKA inactivates myosin light chain kinase by phosphorylating it Myosin light chain kinase phosphorylates myosin light chain, whereas myosin light chain phosphatase dephosphorylates myosin LC-P Accumulation of myosin LC leads to increased relaxation of the airway smooth muscles Myosin LC-P interacts with actin to produce smooth muscle contraction. Since there are low levels of myosin LC-P, contraction is inhibited PKA may also phosphorylate Gq-coupled receptors leading to a cascade of intracellular signals which reduce intracellular Ca2+ Caclium usually binds to calmodulin to activate myosin light chain kinase. Since Ca2+ levles are low, activation of myosin light chain kinase is reduced. Epinephrine can be administered via respiratory inhalation Actin Myosin Actin unbinds from myosin allowing muscle relaxation to occur
PRKAR2B ADRB2 ADCY9 PRKACB PRKAR1B PRKAR2A MYLK ACTB MYL3 MYL3 MYLK CALM1 PPP1CB CALM1 PRKACG PRKAR1A cAMP Eppy Ca2+ Ca2+ ATP Ca2+ Contr Relax O I S P CACNA1A CACNB1 CACNA2D2