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D(2) dopamine receptor Sodium- dependent dopamine transporter Synaptic vesicular amine transporter Voltage- dependent N-type calcium channel subunit alpha-1B Voltage- dependent calcium channel subunit alpha-2/delta-2 Voltage- dependent L-type calcium channel subunit beta-4 Voltage- dependent L-type calcium channel subunit beta-1 Aromatic-L- amino-acid decarboxylase Tyrosine 3-monooxygenase Dopamine Ca+ Dopamine Modafinil Dopamine Ca+ L-Dopa CO2 L-Tyrosine Tetrahydrobiopterin O2 Dihydrobiopterin H2O Pyridoxal 5'-phosphate Gi signaling cascade Fe2+ Inhibition of sleep Presynaptic neuron Post-synaptic neuron 2. An action potential arrives at the nerve terminal and stimulates the opening of the calcium channel, causing an influx of calcium ions 1. Dopamine in synthesized from tyrosine and stored in synaptic vesicles at the nerve terminal 3. Calcium ions stimulates the release of neurotransmitter dopamine into the synaptic cleft via exocytosis 4. Dopamine in the synaptic cleft activates D2 receptors on the 5. Dopamine is taken back up into the presynaptic neuron and recycled 6. modafinil blocks the reuptake transporter, increasing the concentration of dopamine in the synaptic cleft 7. D2 receptor activation activates the Gi signaling cascase, which is an inhibitory pathway that ultimately prevents sleep Synaptic vesicle
DRD2 SLC6A3 SLC18A2 CACNA1B CACNA2D2 CACNB4 CACNB1 DDC TH Dopamine Calcium Dopamine Modafinil Dopamine Calcium L-Dopa Carbon dioxide L-Tyrosine Tetrahydrobiopterin Oxygen Dihydrobiopterin Water Gi signaling cascade
DRD2 SLC6A3 SLC18A2 CACNA1B CACNA2D2 CACNB4 CACNB1 DDC TH LDP Ca+ LDP Provigi LDP Ca+ L-Dopa CO2 Tyr BH4 O2 BH3 H2O Pyr-5'P Gi si c Fe2+ Inhibition of sleep Presynaptic neuron Post-synaptic neuron 2. An action potential arrives at the nerve terminal and stimulates the opening of the calcium channel, causing an influx of calcium ions 1. Dopamine in synthesized from tyrosine and stored in synaptic vesicles at the nerve terminal 3. Calcium ions stimulates the release of neurotransmitter dopamine into the synaptic cleft via exocytosis 4. Dopamine in the synaptic cleft activates D2 receptors on the 5. Dopamine is taken back up into the presynaptic neuron and recycled 6. modafinil blocks the reuptake transporter, increasing the concentration of dopamine in the synaptic cleft 7. D2 receptor activation activates the Gi signaling cascase, which is an inhibitory pathway that ultimately prevents sleep Synaptic vesicle
DRD2 SLC6A3 SLC18A2 CACNA1B CACNA2D2 CACNB4 CACNB1 DDC TH LDP Ca2+ LDP Provigi LDP Ca2+ L-Dopa CO2 Tyr BH4 O2 BH3 H2O Gi si c